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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aneura mirabilis Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP532769BZE
CSB-EP532769BZE
CSB-BP532769BZE
CSB-MP532769BZE
CSB-EP532769BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP532770BZE
CSB-EP532770BZE
CSB-BP532770BZE
CSB-MP532770BZE
CSB-EP532770BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis ATP synthase subunit beta, plastid (atpB) CSB-YP532771BZE
CSB-EP532771BZE
CSB-BP532771BZE
CSB-MP532771BZE
CSB-EP532771BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP532772BZE
CSB-EP532772BZE
CSB-BP532772BZE
CSB-MP532772BZE
CSB-EP532772BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis Plastid 30S ribosomal protein S18 (rps18) CSB-YP532773BZE
CSB-EP532773BZE
CSB-BP532773BZE
CSB-MP532773BZE
CSB-EP532773BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis Translation initiation factor IF-1, plastid (infA) CSB-YP532774BZE
CSB-EP532774BZE
CSB-BP532774BZE
CSB-MP532774BZE
CSB-EP532774BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Maturase K (matK), partial CSB-YP532775ODY
CSB-EP532775ODY
CSB-BP532775ODY
CSB-MP532775ODY
CSB-EP532775ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP532776ODY1
CSB-EP532776ODY1
CSB-BP532776ODY1
CSB-MP532776ODY1
CSB-EP532776ODY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP532777ODY1
CSB-EP532777ODY1
CSB-BP532777ODY1
CSB-MP532777ODY1
CSB-EP532777ODY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Photosystem II reaction center protein I (psbI), partial CSB-YP532778ODY
CSB-EP532778ODY
CSB-BP532778ODY
CSB-MP532778ODY
CSB-EP532778ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Photosystem I reaction center subunit IX (psaJ), partial CSB-YP532779ODY
CSB-EP532779ODY
CSB-BP532779ODY
CSB-MP532779ODY
CSB-EP532779ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP532780ODY
CSB-EP532780ODY
CSB-BP532780ODY
CSB-MP532780ODY
CSB-EP532780ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP532781ODY
CSB-EP532781ODY
CSB-BP532781ODY
CSB-MP532781ODY
CSB-EP532781ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP532782ODY
CSB-EP532782ODY
CSB-BP532782ODY
CSB-MP532782ODY
CSB-EP532782ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP532783ODY1
CSB-EP532783ODY1
CSB-BP532783ODY1
CSB-MP532783ODY1
CSB-EP532783ODY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP532784ODY
CSB-EP532784ODY
CSB-BP532784ODY
CSB-MP532784ODY
CSB-EP532784ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis NAD (P)H-quinone oxidoreductase subunit K, chloroplastic CSB-YP532785ODZ
CSB-EP532785ODZ
CSB-BP532785ODZ
CSB-MP532785ODZ
CSB-EP532785ODZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP532786ODZ
CSB-EP532786ODZ
CSB-BP532786ODZ
CSB-MP532786ODZ
CSB-EP532786ODZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis Chloroplast envelope membrane protein (cemA), partial CSB-YP532787ODZ1
CSB-EP532787ODZ1
CSB-BP532787ODZ1
CSB-MP532787ODZ1
CSB-EP532787ODZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP532788ODZ
CSB-EP532788ODZ
CSB-BP532788ODZ
CSB-MP532788ODZ
CSB-EP532788ODZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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