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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acaryochloris marina Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP534530AVV
CSB-EP534530AVV
CSB-BP534530AVV
CSB-MP534530AVV
CSB-EP534530AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Elongation factor P (efp) CSB-YP534531AVV
CSB-EP534531AVV
CSB-BP534531AVV
CSB-MP534531AVV
CSB-EP534531AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP534532AVV
CSB-EP534532AVV
CSB-BP534532AVV
CSB-MP534532AVV
CSB-EP534532AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP534533AVV
CSB-EP534533AVV
CSB-BP534533AVV
CSB-MP534533AVV
CSB-EP534533AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina 50S ribosomal protein L1 (rplA) CSB-YP534534AVV
CSB-EP534534AVV
CSB-BP534534AVV
CSB-MP534534AVV
CSB-EP534534AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina 50S ribosomal protein L7/L12 (rplL) CSB-YP534535AVV
CSB-EP534535AVV
CSB-BP534535AVV
CSB-MP534535AVV
CSB-EP534535AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina ATP synthase epsilon chain (atpC) CSB-YP534536AVV
CSB-EP534536AVV
CSB-BP534536AVV
CSB-MP534536AVV
CSB-EP534536AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina DNA replication and repair protein recF (recF) CSB-YP534537AVV
CSB-EP534537AVV
CSB-BP534537AVV
CSB-MP534537AVV
CSB-EP534537AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Cytochrome c biogenesis protein CcsA (ccsA), partial CSB-YP534538AVV
CSB-EP534538AVV
CSB-BP534538AVV
CSB-MP534538AVV
CSB-EP534538AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP534539AVV
CSB-EP534539AVV
CSB-BP534539AVV
CSB-MP534539AVV
CSB-EP534539AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP534540AVV
CSB-EP534540AVV
CSB-BP534540AVV
CSB-MP534540AVV
CSB-EP534540AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina tRNA pseudouridine synthase B (truB) CSB-YP534541AVV
CSB-EP534541AVV
CSB-BP534541AVV
CSB-MP534541AVV
CSB-EP534541AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Cytochrome c biogenesis protein CcsB (ccsB), partial CSB-YP534542AVV1
CSB-EP534542AVV1
CSB-BP534542AVV1
CSB-MP534542AVV1
CSB-EP534542AVV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Ribosome-binding factor A (rbfA) CSB-YP534543AVV
CSB-EP534543AVV
CSB-BP534543AVV
CSB-MP534543AVV
CSB-EP534543AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Dihydrodipicolinate reductase (dapB) CSB-YP534544AVV
CSB-EP534544AVV
CSB-BP534544AVV
CSB-MP534544AVV
CSB-EP534544AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina 30S ribosomal protein S21 (rpsU) CSB-YP534545AVV
CSB-EP534545AVV
CSB-BP534545AVV
CSB-MP534545AVV
CSB-EP534545AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina UPF0758 protein AM1_4368 (AM1_4368) CSB-YP534546AVV
CSB-EP534546AVV
CSB-BP534546AVV
CSB-MP534546AVV
CSB-EP534546AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Aspartate--tRNA ligase (aspS), partial CSB-YP534547AVV
CSB-EP534547AVV
CSB-BP534547AVV
CSB-MP534547AVV
CSB-EP534547AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Gamma-glutamyl phosphate reductase (proA) CSB-YP534548AVV
CSB-EP534548AVV
CSB-BP534548AVV
CSB-MP534548AVV
CSB-EP534548AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Proton extrusion protein PcxA (pcxA), partial CSB-YP534549AVV1
CSB-EP534549AVV1
CSB-BP534549AVV1
CSB-MP534549AVV1
CSB-EP534549AVV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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