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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neosartorya fumigata Probable cutinase 3 (AFUB_099910) CSB-YP536530NGT
CSB-EP536530NGT
CSB-BP536530NGT
CSB-MP536530NGT
CSB-EP536530NGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis Photosystem II reaction center protein ycf12 (ycf12), partial CSB-YP536531BZE
CSB-EP536531BZE
CSB-BP536531BZE
CSB-MP536531BZE
CSB-EP536531BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis Maturase K (matK), partial CSB-YP536532BZE
CSB-EP536532BZE
CSB-BP536532BZE
CSB-MP536532BZE
CSB-EP536532BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis 50S ribosomal protein L33, plastid (rpl33) CSB-YP536533BZE
CSB-EP536533BZE
CSB-BP536533BZE
CSB-MP536533BZE
CSB-EP536533BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneura mirabilis 50S ribosomal protein L22, plastid (rpl22) CSB-YP536534BZE
CSB-EP536534BZE
CSB-BP536534BZE
CSB-MP536534BZE
CSB-EP536534BZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP536535ODY
CSB-EP536535ODY
CSB-BP536535ODY
CSB-MP536535ODY
CSB-EP536535ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola NAD (P)H-quinone oxidoreductase subunit K, chloroplastic CSB-YP536536ODY
CSB-EP536536ODY
CSB-BP536536ODY
CSB-MP536536ODY
CSB-EP536536ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP536537ODY
CSB-EP536537ODY
CSB-BP536537ODY
CSB-MP536537ODY
CSB-EP536537ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP536538ODY1
CSB-EP536538ODY1
CSB-BP536538ODY1
CSB-MP536538ODY1
CSB-EP536538ODY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Apocytochrome f (petA), partial CSB-YP536539ODY1
CSB-EP536539ODY1
CSB-BP536539ODY1
CSB-MP536539ODY1
CSB-EP536539ODY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP536540ODY
CSB-EP536540ODY
CSB-BP536540ODY
CSB-MP536540ODY
CSB-EP536540ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP536541ODY
CSB-EP536541ODY
CSB-BP536541ODY
CSB-MP536541ODY
CSB-EP536541ODY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic, partial CSB-YP536542ODY1
CSB-EP536542ODY1
CSB-BP536542ODY1
CSB-MP536542ODY1
CSB-EP536542ODY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis Photosystem Q (B) protein, partial CSB-YP536543ODZ1
CSB-EP536543ODZ1
CSB-BP536543ODZ1
CSB-MP536543ODZ1
CSB-EP536543ODZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP536544ODZ1
CSB-EP536544ODZ1
CSB-BP536544ODZ1
CSB-MP536544ODZ1
CSB-EP536544ODZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis Photosystem II D2 protein (psbD), partial CSB-YP536545ODZ1
CSB-EP536545ODZ1
CSB-BP536545ODZ1
CSB-MP536545ODZ1
CSB-EP536545ODZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP536546ODZ
CSB-EP536546ODZ
CSB-BP536546ODZ
CSB-MP536546ODZ
CSB-EP536546ODZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP536547ODZ
CSB-EP536547ODZ
CSB-BP536547ODZ
CSB-MP536547ODZ
CSB-EP536547ODZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP536548ODZ
CSB-EP536548ODZ
CSB-BP536548ODZ
CSB-MP536548ODZ
CSB-EP536548ODZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis Protein psbN (psbN), partial CSB-YP536549ODZ
CSB-EP536549ODZ
CSB-BP536549ODZ
CSB-MP536549ODZ
CSB-EP536549ODZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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