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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli UPF0253 protein yaeP (yaeP) CSB-YP536790ENP
CSB-EP536790ENP
CSB-BP536790ENP
CSB-MP536790ENP
CSB-EP536790ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Outer membrane protein assembly factor yaeT (yaeT) CSB-YP536791ENP
CSB-EP536791ENP
CSB-BP536791ENP
CSB-MP536791ENP
CSB-EP536791ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP536792ENP
CSB-EP536792ENP
CSB-BP536792ENP
CSB-MP536792ENP
CSB-EP536792ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP536793ENP
CSB-EP536793ENP
CSB-BP536793ENP
CSB-MP536793ENP
CSB-EP536793ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP536794ENP1
CSB-EP536794ENP1
CSB-BP536794ENP1
CSB-MP536794ENP1
CSB-EP536794ENP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome-binding factor A (rbfA) CSB-YP536795ENP
CSB-EP536795ENP
CSB-BP536795ENP
CSB-MP536795ENP
CSB-EP536795ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA pseudouridine synthase B (truB) CSB-YP536796ENP
CSB-EP536796ENP
CSB-BP536796ENP
CSB-MP536796ENP
CSB-EP536796ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Dihydrofolate reductase folM (folM) CSB-YP536797ENP
CSB-EP536797ENP
CSB-BP536797ENP
CSB-MP536797ENP
CSB-EP536797ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Secretion monitor (secM) CSB-YP536798ENP
CSB-EP536798ENP
CSB-BP536798ENP
CSB-MP536798ENP
CSB-EP536798ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribulokinase (araB) CSB-YP536799ENP
CSB-EP536799ENP
CSB-BP536799ENP
CSB-MP536799ENP
CSB-EP536799ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP536800ENP
CSB-EP536800ENP
CSB-BP536800ENP
CSB-MP536800ENP
CSB-EP536800ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable crotonobetaine/carnitine-CoA ligase (caiC) CSB-YP536801ENP
CSB-EP536801ENP
CSB-BP536801ENP
CSB-MP536801ENP
CSB-EP536801ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone protein DnaK (dnaK) CSB-YP536802ENP
CSB-EP536802ENP
CSB-BP536802ENP
CSB-MP536802ENP
CSB-EP536802ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0246 protein yaaA (yaaA) CSB-YP536803ENP
CSB-EP536803ENP
CSB-BP536803ENP
CSB-MP536803ENP
CSB-EP536803ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0597 protein yhaM (yhaM) CSB-YP536804ENP
CSB-EP536804ENP
CSB-BP536804ENP
CSB-MP536804ENP
CSB-EP536804ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S21 (rpsU) CSB-YP536805ENP
CSB-EP536805ENP
CSB-BP536805ENP
CSB-MP536805ENP
CSB-EP536805ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0056 inner membrane protein marC (marC), partial CSB-YP536806ENP1
CSB-EP536806ENP1
CSB-BP536806ENP1
CSB-MP536806ENP1
CSB-EP536806ENP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Autoinducer 2-degrading protein lsrG (lsrG) CSB-YP536807ENP
CSB-EP536807ENP
CSB-BP536807ENP
CSB-MP536807ENP
CSB-EP536807ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Autoinducer 2 import system permease protein lsrD (lsrD), partial CSB-YP536808ENP
CSB-EP536808ENP
CSB-BP536808ENP
CSB-MP536808ENP
CSB-EP536808ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Autoinducer 2 import system permease protein lsrC (lsrC), partial CSB-YP536809ENP
CSB-EP536809ENP
CSB-BP536809ENP
CSB-MP536809ENP
CSB-EP536809ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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