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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Streptomyces griseus subsp. griseus Putative phenylalanine aminotransferase (pat) CSB-YP540250FOF
CSB-EP540250FOF
CSB-BP540250FOF
CSB-MP540250FOF
CSB-EP540250FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L34 (rpmH) CSB-YP540251FOF
CSB-EP540251FOF
CSB-BP540251FOF
CSB-MP540251FOF
CSB-EP540251FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S14 (rpsN) CSB-YP540252FOF
CSB-EP540252FOF
CSB-BP540252FOF
CSB-MP540252FOF
CSB-EP540252FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Mycothiol acetyltransferase (mshD) CSB-YP540253FOF
CSB-EP540253FOF
CSB-BP540253FOF
CSB-MP540253FOF
CSB-EP540253FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP540254FOF
CSB-EP540254FOF
CSB-BP540254FOF
CSB-MP540254FOF
CSB-EP540254FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP540255FOF
CSB-EP540255FOF
CSB-BP540255FOF
CSB-MP540255FOF
CSB-EP540255FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Arginine--tRNA ligase (argS) CSB-YP540256FOF
CSB-EP540256FOF
CSB-BP540256FOF
CSB-MP540256FOF
CSB-EP540256FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus D-ribose pyranase 2 (rbsD2) CSB-YP540257FOF
CSB-EP540257FOF
CSB-BP540257FOF
CSB-MP540257FOF
CSB-EP540257FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Imidazolonepropionase (hutI) CSB-YP540258FOF
CSB-EP540258FOF
CSB-BP540258FOF
CSB-MP540258FOF
CSB-EP540258FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Peptide chain release factor 2 (prfB) CSB-YP540259FOF
CSB-EP540259FOF
CSB-BP540259FOF
CSB-MP540259FOF
CSB-EP540259FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP540260FOF
CSB-EP540260FOF
CSB-BP540260FOF
CSB-MP540260FOF
CSB-EP540260FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP540261FOF
CSB-EP540261FOF
CSB-BP540261FOF
CSB-MP540261FOF
CSB-EP540261FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable rRNA maturation factor (SGR_5003) CSB-YP540262FOF
CSB-EP540262FOF
CSB-BP540262FOF
CSB-MP540262FOF
CSB-EP540262FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP540263FOF
CSB-EP540263FOF
CSB-BP540263FOF
CSB-MP540263FOF
CSB-EP540263FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP540264FOF
CSB-EP540264FOF
CSB-BP540264FOF
CSB-MP540264FOF
CSB-EP540264FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable cytosol aminopeptidase (pepA), partial CSB-YP540265FOF
CSB-EP540265FOF
CSB-BP540265FOF
CSB-MP540265FOF
CSB-EP540265FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus UPF0286 protein SGR_2149 (SGR_2149) CSB-YP540266FOF
CSB-EP540266FOF
CSB-BP540266FOF
CSB-MP540266FOF
CSB-EP540266FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Tryptophan synthase beta chain (trpB) CSB-YP540267FOF
CSB-EP540267FOF
CSB-BP540267FOF
CSB-MP540267FOF
CSB-EP540267FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable 5-dehydro-4-deoxyglucarate dehydratase (SGR_5617) CSB-YP540268FOF
CSB-EP540268FOF
CSB-BP540268FOF
CSB-MP540268FOF
CSB-EP540268FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP540269FOF
CSB-EP540269FOF
CSB-BP540269FOF
CSB-MP540269FOF
CSB-EP540269FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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