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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli N-methyl-L-tryptophan oxidase (solA) CSB-YP542334ENT
CSB-EP542334ENT
CSB-BP542334ENT
CSB-MP542334ENT
CSB-EP542334ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate--tRNA ligase (gltX) CSB-YP542335ENT
CSB-EP542335ENT
CSB-BP542335ENT
CSB-MP542335ENT
CSB-EP542335ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multidrug resistance protein MdtL (mdtL), partial CSB-YP542336ENT
CSB-EP542336ENT
CSB-BP542336ENT
CSB-MP542336ENT
CSB-EP542336ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP synthase gamma chain (atpG) CSB-YP542337ENT
CSB-EP542337ENT
CSB-BP542337ENT
CSB-MP542337ENT
CSB-EP542337ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate--ammonia ligase (asnA) CSB-YP542338ENT
CSB-EP542338ENT
CSB-BP542338ENT
CSB-MP542338ENT
CSB-EP542338ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator hdfR (hdfR) CSB-YP542339ENT
CSB-EP542339ENT
CSB-BP542339ENT
CSB-MP542339ENT
CSB-EP542339ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent RNA helicase rhlB (rhlB) CSB-YP542340ENT
CSB-EP542340ENT
CSB-BP542340ENT
CSB-MP542340ENT
CSB-EP542340ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP542341ENT
CSB-EP542341ENT
CSB-BP542341ENT
CSB-MP542341ENT
CSB-EP542341ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0745 protein YcgL (ycgL) CSB-YP542342ENT
CSB-EP542342ENT
CSB-BP542342ENT
CSB-MP542342ENT
CSB-EP542342ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA ligase (ligA) CSB-YP542343ENT
CSB-EP542343ENT
CSB-BP542343ENT
CSB-MP542343ENT
CSB-EP542343ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator murR (murR) CSB-YP542344ENT
CSB-EP542344ENT
CSB-BP542344ENT
CSB-MP542344ENT
CSB-EP542344ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GDP-mannose pyrophosphatase nudK (nudK) CSB-YP542345ENT
CSB-EP542345ENT
CSB-BP542345ENT
CSB-MP542345ENT
CSB-EP542345ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP542346ENT
CSB-EP542346ENT
CSB-BP542346ENT
CSB-MP542346ENT
CSB-EP542346ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) CSB-YP542347ENT
CSB-EP542347ENT
CSB-BP542347ENT
CSB-MP542347ENT
CSB-EP542347ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptidyl-tRNA hydrolase (pth) CSB-YP542348ENT
CSB-EP542348ENT
CSB-BP542348ENT
CSB-MP542348ENT
CSB-EP542348ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamyl-tRNA reductase (hemA) CSB-YP542349ENT
CSB-EP542349ENT
CSB-BP542349ENT
CSB-MP542349ENT
CSB-EP542349ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP542350ENT
CSB-EP542350ENT
CSB-BP542350ENT
CSB-MP542350ENT
CSB-EP542350ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP542351ENT
CSB-EP542351ENT
CSB-BP542351ENT
CSB-MP542351ENT
CSB-EP542351ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptidase B (pepB) CSB-YP542352ENT
CSB-EP542352ENT
CSB-BP542352ENT
CSB-MP542352ENT
CSB-EP542352ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-rhamnose mutarotase (rhaM) CSB-YP542353ENT
CSB-EP542353ENT
CSB-BP542353ENT
CSB-MP542353ENT
CSB-EP542353ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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