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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Streptomyces griseus subsp. griseus 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (mshB) CSB-YP542894FOF
CSB-EP542894FOF
CSB-BP542894FOF
CSB-MP542894FOF
CSB-EP542894FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus L-ectoine synthase (ectC) CSB-YP542895FOF
CSB-EP542895FOF
CSB-BP542895FOF
CSB-MP542895FOF
CSB-EP542895FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Putative NADH dehydrogenase/NAD (P)H nitroreductase SGR_2476 CSB-YP542896FOF
CSB-EP542896FOF
CSB-BP542896FOF
CSB-MP542896FOF
CSB-EP542896FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP542897FOF
CSB-EP542897FOF
CSB-BP542897FOF
CSB-MP542897FOF
CSB-EP542897FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L35 (rpmI) CSB-YP542898FOF
CSB-EP542898FOF
CSB-BP542898FOF
CSB-MP542898FOF
CSB-EP542898FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP542899FOF
CSB-EP542899FOF
CSB-BP542899FOF
CSB-MP542899FOF
CSB-EP542899FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Alanine--tRNA ligase (alaS), partial CSB-YP542900FOF
CSB-EP542900FOF
CSB-BP542900FOF
CSB-MP542900FOF
CSB-EP542900FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Phosphoglucosamine mutase (glmM) CSB-YP542901FOF
CSB-EP542901FOF
CSB-BP542901FOF
CSB-MP542901FOF
CSB-EP542901FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S11 (rpsK) CSB-YP542902FOF
CSB-EP542902FOF
CSB-BP542902FOF
CSB-MP542902FOF
CSB-EP542902FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L30 (rpmD) CSB-YP542903FOF
CSB-EP542903FOF
CSB-BP542903FOF
CSB-MP542903FOF
CSB-EP542903FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S8 (rpsH) CSB-YP542904FOF
CSB-EP542904FOF
CSB-BP542904FOF
CSB-MP542904FOF
CSB-EP542904FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L29 (rpmC) CSB-YP542905FOF
CSB-EP542905FOF
CSB-BP542905FOF
CSB-MP542905FOF
CSB-EP542905FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S12 (rpsL) CSB-YP542906FOF
CSB-EP542906FOF
CSB-BP542906FOF
CSB-MP542906FOF
CSB-EP542906FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP542907FOF
CSB-EP542907FOF
CSB-BP542907FOF
CSB-MP542907FOF
CSB-EP542907FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus NADH-quinone oxidoreductase subunit N 2 (nuoN2), partial CSB-YP542908FOF
CSB-EP542908FOF
CSB-BP542908FOF
CSB-MP542908FOF
CSB-EP542908FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus NADH-quinone oxidoreductase subunit D 1 (nuoD1) CSB-YP542909FOF
CSB-EP542909FOF
CSB-BP542909FOF
CSB-MP542909FOF
CSB-EP542909FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Catalase-peroxidase (katG), partial CSB-YP542910FOF
CSB-EP542910FOF
CSB-BP542910FOF
CSB-MP542910FOF
CSB-EP542910FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein T (psbT), partial CSB-YP542911DZY
CSB-EP542911DZY
CSB-BP542911DZY
CSB-MP542911DZY
CSB-EP542911DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Elongation factor P (efp) CSB-YP542912DZY
CSB-EP542912DZY
CSB-BP542912DZY
CSB-MP542912DZY
CSB-EP542912DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Homoserine kinase (thrB) CSB-YP542913DZY
CSB-EP542913DZY
CSB-BP542913DZY
CSB-MP542913DZY
CSB-EP542913DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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