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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomyces griseus subsp. griseus Ribosome maturation factor RimM (rimM) CSB-YP543554FOF
CSB-EP543554FOF
CSB-BP543554FOF
CSB-MP543554FOF
CSB-EP543554FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus D-alanine--D-alanine ligase (ddl) CSB-YP543555FOF
CSB-EP543555FOF
CSB-BP543555FOF
CSB-MP543555FOF
CSB-EP543555FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Glutamate--tRNA ligase (gltX), partial CSB-YP543556FOF
CSB-EP543556FOF
CSB-BP543556FOF
CSB-MP543556FOF
CSB-EP543556FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus DNA ligase 2 (ligA2), partial CSB-YP543557FOF
CSB-EP543557FOF
CSB-BP543557FOF
CSB-MP543557FOF
CSB-EP543557FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus ATP synthase gamma chain (atpG) CSB-YP543558FOF
CSB-EP543558FOF
CSB-BP543558FOF
CSB-MP543558FOF
CSB-EP543558FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP543559FOF
CSB-EP543559FOF
CSB-BP543559FOF
CSB-MP543559FOF
CSB-EP543559FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Phosphoglycerate kinase (pgk) CSB-YP543560FOF
CSB-EP543560FOF
CSB-BP543560FOF
CSB-MP543560FOF
CSB-EP543560FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Sec-independent protein translocase protein TatB (tatB), partial CSB-YP543561FOF
CSB-EP543561FOF
CSB-BP543561FOF
CSB-MP543561FOF
CSB-EP543561FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Putative 3-methyladenine DNA glycosylase (SGR_5705) CSB-YP543562FOF
CSB-EP543562FOF
CSB-BP543562FOF
CSB-MP543562FOF
CSB-EP543562FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP543563FOF
CSB-EP543563FOF
CSB-BP543563FOF
CSB-MP543563FOF
CSB-EP543563FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Proteasome subunit alpha (prcA) CSB-YP543564FOF
CSB-EP543564FOF
CSB-BP543564FOF
CSB-MP543564FOF
CSB-EP543564FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Acetylglutamate kinase (argB) CSB-YP543565FOF
CSB-EP543565FOF
CSB-BP543565FOF
CSB-MP543565FOF
CSB-EP543565FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP543566FOF
CSB-EP543566FOF
CSB-BP543566FOF
CSB-MP543566FOF
CSB-EP543566FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP543567FOF
CSB-EP543567FOF
CSB-BP543567FOF
CSB-MP543567FOF
CSB-EP543567FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S9 (rpsI) CSB-YP543568FOF
CSB-EP543568FOF
CSB-BP543568FOF
CSB-MP543568FOF
CSB-EP543568FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L36 (rpmJ) CSB-YP543569FOF
CSB-EP543569FOF
CSB-BP543569FOF
CSB-MP543569FOF
CSB-EP543569FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L6 (rplF) CSB-YP543570FOF
CSB-EP543570FOF
CSB-BP543570FOF
CSB-MP543570FOF
CSB-EP543570FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S14 type Z (rpsZ) CSB-YP543571FOF
CSB-EP543571FOF
CSB-BP543571FOF
CSB-MP543571FOF
CSB-EP543571FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L16 (rplP) CSB-YP543572FOF
CSB-EP543572FOF
CSB-BP543572FOF
CSB-MP543572FOF
CSB-EP543572FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L2 (rplB) CSB-YP543573FOF
CSB-EP543573FOF
CSB-BP543573FOF
CSB-MP543573FOF
CSB-EP543573FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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