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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aspergillus terreus Ubiquinone biosynthesis protein coq4, mitochondrial (coq4) CSB-YP602333APT
CSB-EP602333APT
CSB-BP602333APT
CSB-MP602333APT
CSB-EP602333APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus U3 small nucleolar RNA-associated protein 25 (utp25), partial CSB-YP602334APT
CSB-EP602334APT
CSB-BP602334APT
CSB-MP602334APT
CSB-EP602334APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Long chronological lifespan protein 2 (lcl2) CSB-YP602335APT
CSB-EP602335APT
CSB-BP602335APT
CSB-MP602335APT
CSB-EP602335APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Mitochondrial thiamine pyrophosphate carrier 1 (tpc1), partial CSB-YP602336APT1
CSB-EP602336APT1
CSB-BP602336APT1
CSB-MP602336APT1
CSB-EP602336APT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable beta-glucosidase btgE (btgE), partial CSB-YP602337APT
CSB-EP602337APT
CSB-BP602337APT
CSB-MP602337APT
CSB-EP602337APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable pectin lyase F-1 (pelF-1) CSB-YP602338APT
CSB-EP602338APT
CSB-BP602338APT
CSB-MP602338APT
CSB-EP602338APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable exopolygalacturonase X (pgaX) CSB-YP602339APT
CSB-EP602339APT
CSB-BP602339APT
CSB-MP602339APT
CSB-EP602339APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Protein transport protein sec13 (sec13) CSB-YP602340APT
CSB-EP602340APT
CSB-BP602340APT
CSB-MP602340APT
CSB-EP602340APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable beta-mannosidase A (mndA), partial CSB-YP602341APT
CSB-EP602341APT
CSB-BP602341APT
CSB-MP602341APT
CSB-EP602341APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus ATP-dependent rRNA helicase rrp3 (rrp3) CSB-YP602342APT
CSB-EP602342APT
CSB-BP602342APT
CSB-MP602342APT
CSB-EP602342APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Mediator of RNA polymerase II transcription subunit 7 (med7) CSB-YP602343APT
CSB-EP602343APT
CSB-BP602343APT
CSB-MP602343APT
CSB-EP602343APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable HECT-type ubiquitin ligase-interacting protein creD (creD), partial CSB-YP602344APT
CSB-EP602344APT
CSB-BP602344APT
CSB-MP602344APT
CSB-EP602344APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Cytokinesis protein sepH (sepH), partial CSB-YP602345APT
CSB-EP602345APT
CSB-BP602345APT
CSB-MP602345APT
CSB-EP602345APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus ATP-dependent RNA helicase ded1 (ded1), partial CSB-YP602346APT
CSB-EP602346APT
CSB-BP602346APT
CSB-MP602346APT
CSB-EP602346APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Pescadillo homolog (nop7), partial CSB-YP602347APT
CSB-EP602347APT
CSB-BP602347APT
CSB-MP602347APT
CSB-EP602347APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Adenylosuccinate synthetase (ATEG_05239) CSB-YP602348APT
CSB-EP602348APT
CSB-BP602348APT
CSB-MP602348APT
CSB-EP602348APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus ATP-dependent RNA helicase dbp4 (dbp4), partial CSB-YP602349APT
CSB-EP602349APT
CSB-BP602349APT
CSB-MP602349APT
CSB-EP602349APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Eukaryotic translation initiation factor 3 subunit B (prt1), partial CSB-YP602350APT
CSB-EP602350APT
CSB-BP602350APT
CSB-MP602350APT
CSB-EP602350APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Pyruvate decarboxylase (pdcA), partial CSB-YP602351APT
CSB-EP602351APT
CSB-BP602351APT
CSB-MP602351APT
CSB-EP602351APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Stress response protein nst1 (nst1), partial CSB-YP602352APT
CSB-EP602352APT
CSB-BP602352APT
CSB-MP602352APT
CSB-EP602352APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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