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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aspergillus terreus Protein clp1 (clp1), partial CSB-YP602633APT
CSB-EP602633APT
CSB-BP602633APT
CSB-MP602633APT
CSB-EP602633APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Methylthioribose-1-phosphate isomerase (mri1) CSB-YP602634APT
CSB-EP602634APT
CSB-BP602634APT
CSB-MP602634APT
CSB-EP602634APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus ATP-dependent RNA helicase sub2 (sub2) CSB-YP602635APT
CSB-EP602635APT
CSB-BP602635APT
CSB-MP602635APT
CSB-EP602635APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus ATP-dependent RNA helicase mak5 (mak5), partial CSB-YP602636APT
CSB-EP602636APT
CSB-BP602636APT
CSB-MP602636APT
CSB-EP602636APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable D-xylulose kinase A (xkiA) CSB-YP602637APT
CSB-EP602637APT
CSB-BP602637APT
CSB-MP602637APT
CSB-EP602637APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Protein rot1 (rot1), partial CSB-YP602638APT
CSB-EP602638APT
CSB-BP602638APT
CSB-MP602638APT
CSB-EP602638APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus PAB-dependent poly (A)-specific ribonuclease subunit pan2 (pan2), partial CSB-YP602639APT
CSB-EP602639APT
CSB-BP602639APT
CSB-MP602639APT
CSB-EP602639APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Sterol 3-beta-glucosyltransferase (atg26), partial CSB-YP602640APT
CSB-EP602640APT
CSB-BP602640APT
CSB-MP602640APT
CSB-EP602640APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable pectate lyase C (plyC) CSB-YP602641APT
CSB-EP602641APT
CSB-BP602641APT
CSB-MP602641APT
CSB-EP602641APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable beta-galactosidase B (lacB), partial CSB-YP602642APT
CSB-EP602642APT
CSB-BP602642APT
CSB-MP602642APT
CSB-EP602642APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Lysophospholipase nte1 (nte1), partial CSB-YP602643APT
CSB-EP602643APT
CSB-BP602643APT
CSB-MP602643APT
CSB-EP602643APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Eukaryotic translation initiation factor 3 subunit M (ATEG_04279) CSB-YP602644APT
CSB-EP602644APT
CSB-BP602644APT
CSB-MP602644APT
CSB-EP602644APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei Glycerol kinase (glpK) CSB-YP602645AAAL
CSB-EP602645AAAL
CSB-BP602645AAAL
CSB-MP602645AAAL
CSB-EP602645AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei Tryptophan synthase alpha chain (trpA) CSB-YP602646AAAL
CSB-EP602646AAAL
CSB-BP602646AAAL
CSB-MP602646AAAL
CSB-EP602646AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei Zinc import ATP-binding protein ZnuC (znuC) CSB-YP602647AAAL
CSB-EP602647AAAL
CSB-BP602647AAAL
CSB-MP602647AAAL
CSB-EP602647AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei Phosphate import ATP-binding protein PstB (pstB) CSB-YP602648AAAL
CSB-EP602648AAAL
CSB-BP602648AAAL
CSB-MP602648AAAL
CSB-EP602648AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP602649AAAL
CSB-EP602649AAAL
CSB-BP602649AAAL
CSB-MP602649AAAL
CSB-EP602649AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP602650AAAL
CSB-EP602650AAAL
CSB-BP602650AAAL
CSB-MP602650AAAL
CSB-EP602650AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP602651AAAL
CSB-EP602651AAAL
CSB-BP602651AAAL
CSB-MP602651AAAL
CSB-EP602651AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkalilimnicola ehrlichei GTPase obg (obg) CSB-YP602652AAAL
CSB-EP602652AAAL
CSB-BP602652AAAL
CSB-MP602652AAAL
CSB-EP602652AAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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