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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aspergillus terreus Hsp70 nucleotide exchange factor fes1 (fes1) CSB-YP603513APT
CSB-EP603513APT
CSB-BP603513APT
CSB-MP603513APT
CSB-EP603513APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus GPI inositol-deacylase (bst1), partial CSB-YP603514APT
CSB-EP603514APT
CSB-BP603514APT
CSB-MP603514APT
CSB-EP603514APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable alpha-glucuronidase A (aguA), partial CSB-YP603515APT
CSB-EP603515APT
CSB-BP603515APT
CSB-MP603515APT
CSB-EP603515APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Polynucleotide 5'-hydroxyl-kinase grc3 (grc3), partial CSB-YP603516APT
CSB-EP603516APT
CSB-BP603516APT
CSB-MP603516APT
CSB-EP603516APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Protein get1 (get1), partial CSB-YP603517APT1
CSB-EP603517APT1
CSB-BP603517APT1
CSB-MP603517APT1
CSB-EP603517APT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Methionine aminopeptidase 2 homolog ATEG_05540 (ATEG_05540) CSB-YP603518APT
CSB-EP603518APT
CSB-BP603518APT
CSB-MP603518APT
CSB-EP603518APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus ATPase get3 (get3) CSB-YP603519APT
CSB-EP603519APT
CSB-BP603519APT
CSB-MP603519APT
CSB-EP603519APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Eukaryotic translation initiation factor 3 subunit L (ATEG_04476) CSB-YP603520APT
CSB-EP603520APT
CSB-BP603520APT
CSB-MP603520APT
CSB-EP603520APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus DNA mismatch repair protein msh3 (msh3), partial CSB-YP603521APT
CSB-EP603521APT
CSB-BP603521APT
CSB-MP603521APT
CSB-EP603521APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Polyadenylate-binding protein, cytoplasmic and nuclear (pab1), partial CSB-YP603522APT
CSB-EP603522APT
CSB-BP603522APT
CSB-MP603522APT
CSB-EP603522APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus U3 small nucleolar RNA-associated protein 10 (utp10), partial CSB-YP603523APT
CSB-EP603523APT
CSB-BP603523APT
CSB-MP603523APT
CSB-EP603523APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Golgi apparatus membrane protein tvp38 (tvp38), partial CSB-YP603524APT1
CSB-EP603524APT1
CSB-BP603524APT1
CSB-MP603524APT1
CSB-EP603524APT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus tRNA (guanine-N (7)-)-methyltransferase (trm8) CSB-YP603525APT
CSB-EP603525APT
CSB-BP603525APT
CSB-MP603525APT
CSB-EP603525APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable alpha-N-arabinofuranosidase A (abfA), partial CSB-YP603526APT
CSB-EP603526APT
CSB-BP603526APT
CSB-MP603526APT
CSB-EP603526APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Patatin-like phospholipase domain-containing protein ATEG_02594 (ATEG_02594), partial CSB-YP603527APT1
CSB-EP603527APT1
CSB-BP603527APT1
CSB-MP603527APT1
CSB-EP603527APT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Autophagy-related protein 18 (atg18) CSB-YP603528APT
CSB-EP603528APT
CSB-BP603528APT
CSB-MP603528APT
CSB-EP603528APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Eukaryotic translation initiation factor 3 subunit I (tif34) CSB-YP603529APT
CSB-EP603529APT
CSB-BP603529APT
CSB-MP603529APT
CSB-EP603529APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Probable E3 ubiquitin ligase complex SCF subunit sconB (sconB), partial CSB-YP603530APT
CSB-EP603530APT
CSB-BP603530APT
CSB-MP603530APT
CSB-EP603530APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Protein fmp52-2, mitochondrial (fmp522) CSB-YP603531APT
CSB-EP603531APT
CSB-BP603531APT
CSB-MP603531APT
CSB-EP603531APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus terreus Kynureninase 2 (bna5-2) CSB-YP603532APT
CSB-EP603532APT
CSB-BP603532APT
CSB-MP603532APT
CSB-EP603532APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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