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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium perfringens Phosphomethylpyrimidine synthase (thiC) CSB-YP605695CAAN
CSB-EP605695CAAN
CSB-BP605695CAAN
CSB-MP605695CAAN
CSB-EP605695CAAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens Glutamine--tRNA ligase (glnS), partial CSB-YP605696CAAN
CSB-EP605696CAAN
CSB-BP605696CAAN
CSB-MP605696CAAN
CSB-EP605696CAAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens Histidine decarboxylase proenzyme (hdc) CSB-YP605697CAAN
CSB-EP605697CAAN
CSB-BP605697CAAN
CSB-MP605697CAAN
CSB-EP605697CAAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens UvrABC system protein C (uvrC), partial CSB-YP605698CAAN
CSB-EP605698CAAN
CSB-BP605698CAAN
CSB-MP605698CAAN
CSB-EP605698CAAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens Arginine deiminase (arcA) CSB-YP605699CAAN
CSB-EP605699CAAN
CSB-BP605699CAAN
CSB-MP605699CAAN
CSB-EP605699CAAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD), partial CSB-YP605700CAAN
CSB-EP605700CAAN
CSB-BP605700CAAN
CSB-MP605700CAAN
CSB-EP605700CAAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens Peptidase T (pepT) CSB-YP605701CAAN
CSB-EP605701CAAN
CSB-BP605701CAAN
CSB-MP605701CAAN
CSB-EP605701CAAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Vacuolar protein sorting/targeting protein 10 (VPS10), partial CSB-YP605702EUG
CSB-EP605702EUG
CSB-BP605702EUG
CSB-MP605702EUG
CSB-EP605702EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Required for respiratory growth protein 9, mitochondrial (RRG9) CSB-YP605703EUG
CSB-EP605703EUG
CSB-BP605703EUG
CSB-MP605703EUG
CSB-EP605703EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum tRNA (guanine-N (7)-)-methyltransferase (TRM8) CSB-YP605704EUG
CSB-EP605704EUG
CSB-BP605704EUG
CSB-MP605704EUG
CSB-EP605704EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Histone-lysine N-methyltransferase SET9 (SET9), partial CSB-YP605705EUG
CSB-EP605705EUG
CSB-BP605705EUG
CSB-MP605705EUG
CSB-EP605705EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Mannitol-1-phosphate 5-dehydrogenase (mpd1) CSB-YP605706EUG
CSB-EP605706EUG
CSB-BP605706EUG
CSB-MP605706EUG
CSB-EP605706EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Amino-acid acetyltransferase, mitochondrial (ARG2), partial CSB-YP605707EUG
CSB-EP605707EUG
CSB-BP605707EUG
CSB-MP605707EUG
CSB-EP605707EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Vacuolar fusion protein MON1 (MON1), partial CSB-YP605708EUG
CSB-EP605708EUG
CSB-BP605708EUG
CSB-MP605708EUG
CSB-EP605708EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum DNA ligase 4 (LIG4), partial CSB-YP605709EUG
CSB-EP605709EUG
CSB-BP605709EUG
CSB-MP605709EUG
CSB-EP605709EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) CSB-YP605710EUG
CSB-EP605710EUG
CSB-BP605710EUG
CSB-MP605710EUG
CSB-EP605710EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Eukaryotic translation initiation factor 3 subunit L (SNOG_08318) CSB-YP605711EUG
CSB-EP605711EUG
CSB-BP605711EUG
CSB-MP605711EUG
CSB-EP605711EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Nascent polypeptide-associated complex subunit beta (EGD1) CSB-YP605712EUG
CSB-EP605712EUG
CSB-BP605712EUG
CSB-MP605712EUG
CSB-EP605712EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Protein transport protein SEC13 (SEC13) CSB-YP605713EUG
CSB-EP605713EUG
CSB-BP605713EUG
CSB-MP605713EUG
CSB-EP605713EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum ATPase GET3 (GET3) CSB-YP605714EUG
CSB-EP605714EUG
CSB-BP605714EUG
CSB-MP605714EUG
CSB-EP605714EUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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