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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella flexneri serotype 5b Asparagine--tRNA ligase (asnS) CSB-YP611519SGG
CSB-EP611519SGG
CSB-BP611519SGG
CSB-MP611519SGG
CSB-EP611519SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b UPF0194 membrane protein ybhG (ybhG) CSB-YP611520SGG
CSB-EP611520SGG
CSB-BP611520SGG
CSB-MP611520SGG
CSB-EP611520SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Lipoyl synthase (lipA) CSB-YP611521SGG
CSB-EP611521SGG
CSB-BP611521SGG
CSB-MP611521SGG
CSB-EP611521SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Probable rRNA maturation factor YbeY (ybeY) CSB-YP611522SGG
CSB-EP611522SGG
CSB-BP611522SGG
CSB-MP611522SGG
CSB-EP611522SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP611523SGG
CSB-EP611523SGG
CSB-BP611523SGG
CSB-MP611523SGG
CSB-EP611523SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Probable 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (citG) CSB-YP611524SGG
CSB-EP611524SGG
CSB-BP611524SGG
CSB-MP611524SGG
CSB-EP611524SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP611525SGG
CSB-EP611525SGG
CSB-BP611525SGG
CSB-MP611525SGG
CSB-EP611525SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP611526SGG
CSB-EP611526SGG
CSB-BP611526SGG
CSB-MP611526SGG
CSB-EP611526SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP611527SGG
CSB-EP611527SGG
CSB-BP611527SGG
CSB-MP611527SGG
CSB-EP611527SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Proline--tRNA ligase (proS), partial CSB-YP611528SGG
CSB-EP611528SGG
CSB-BP611528SGG
CSB-MP611528SGG
CSB-EP611528SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Outer membrane protein assembly factor YaeT (yaeT), partial CSB-YP611529SGG
CSB-EP611529SGG
CSB-BP611529SGG
CSB-MP611529SGG
CSB-EP611529SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b [Protein-PII] uridylyltransferase (glnD), partial CSB-YP611530SGG
CSB-EP611530SGG
CSB-BP611530SGG
CSB-MP611530SGG
CSB-EP611530SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP611531SGG
CSB-EP611531SGG
CSB-BP611531SGG
CSB-MP611531SGG
CSB-EP611531SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b UPF0289 protein yacF (yacF) CSB-YP611532SGG
CSB-EP611532SGG
CSB-BP611532SGG
CSB-MP611532SGG
CSB-EP611532SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Protein MraZ (mraZ) CSB-YP611533SGG
CSB-EP611533SGG
CSB-BP611533SGG
CSB-MP611533SGG
CSB-EP611533SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b L-arabinose isomerase (araA) CSB-YP611534SGG
CSB-EP611534SGG
CSB-BP611534SGG
CSB-MP611534SGG
CSB-EP611534SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP611535SGG
CSB-EP611535SGG
CSB-BP611535SGG
CSB-MP611535SGG
CSB-EP611535SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP611536SGG
CSB-EP611536SGG
CSB-BP611536SGG
CSB-MP611536SGG
CSB-EP611536SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Chaperone protein DnaJ (dnaJ) CSB-YP611537SGG
CSB-EP611537SGG
CSB-BP611537SGG
CSB-MP611537SGG
CSB-EP611537SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC (rlmC) CSB-YP611538SGG
CSB-EP611538SGG
CSB-BP611538SGG
CSB-MP611538SGG
CSB-EP611538SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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