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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella flexneri serotype 5b Fumarate reductase subunit D (frdD), partial CSB-YP612841SGG1
CSB-EP612841SGG1
CSB-BP612841SGG1
CSB-MP612841SGG1
CSB-EP612841SGG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP612842SGG
CSB-EP612842SGG
CSB-BP612842SGG
CSB-MP612842SGG
CSB-EP612842SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b N-acetylneuraminate epimerase (nanM) CSB-YP612843SGG
CSB-EP612843SGG
CSB-BP612843SGG
CSB-MP612843SGG
CSB-EP612843SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Maltoporin (lamB) CSB-YP612844SGG
CSB-EP612844SGG
CSB-BP612844SGG
CSB-MP612844SGG
CSB-EP612844SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Homoserine O-succinyltransferase (metA) CSB-YP612845SGG
CSB-EP612845SGG
CSB-BP612845SGG
CSB-MP612845SGG
CSB-EP612845SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 50S ribosomal protein L7/L12 (rplL) CSB-YP612846SGG
CSB-EP612846SGG
CSB-BP612846SGG
CSB-MP612846SGG
CSB-EP612846SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP612847SGG
CSB-EP612847SGG
CSB-BP612847SGG
CSB-MP612847SGG
CSB-EP612847SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP612848SGG
CSB-EP612848SGG
CSB-BP612848SGG
CSB-MP612848SGG
CSB-EP612848SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP612849SGG
CSB-EP612849SGG
CSB-BP612849SGG
CSB-MP612849SGG
CSB-EP612849SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Nucleoid occlusion factor SlmA (slmA) CSB-YP612850SGG
CSB-EP612850SGG
CSB-BP612850SGG
CSB-MP612850SGG
CSB-EP612850SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Putative transport protein YidE (yidE), partial CSB-YP612851SGG
CSB-EP612851SGG
CSB-BP612851SGG
CSB-MP612851SGG
CSB-EP612851SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b ATPase ravA (ravA) CSB-YP612852SGG
CSB-EP612852SGG
CSB-BP612852SGG
CSB-MP612852SGG
CSB-EP612852SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b ATP synthase subunit c (atpE), partial CSB-YP612853SGG1
CSB-EP612853SGG1
CSB-BP612853SGG1
CSB-MP612853SGG1
CSB-EP612853SGG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b HTH-type transcriptional regulator hdfR (hdfR) CSB-YP612854SGG
CSB-EP612854SGG
CSB-BP612854SGG
CSB-MP612854SGG
CSB-EP612854SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP612855SGG
CSB-EP612855SGG
CSB-BP612855SGG
CSB-MP612855SGG
CSB-EP612855SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Sec-independent protein translocase protein TatB (tatB), partial CSB-YP612856SGG
CSB-EP612856SGG
CSB-BP612856SGG
CSB-MP612856SGG
CSB-EP612856SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 3-ketoacyl-CoA thiolase (fadA) CSB-YP612857SGG
CSB-EP612857SGG
CSB-BP612857SGG
CSB-MP612857SGG
CSB-EP612857SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b HTH-type transcriptional activator rhaS (rhaS) CSB-YP612858SGG
CSB-EP612858SGG
CSB-BP612858SGG
CSB-MP612858SGG
CSB-EP612858SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b C4-dicarboxylate transport protein (dctA), partial CSB-YP612859SGG
CSB-EP612859SGG
CSB-BP612859SGG
CSB-MP612859SGG
CSB-EP612859SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Nickel import ATP-binding protein NikD (nikD) CSB-YP612860SGG
CSB-EP612860SGG
CSB-BP612860SGG
CSB-MP612860SGG
CSB-EP612860SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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