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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia xenovorans 50S ribosomal protein L5 (rplE) CSB-YP614484BNV
CSB-EP614484BNV
CSB-BP614484BNV
CSB-MP614484BNV
CSB-EP614484BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 50S ribosomal protein L18 (rplR) CSB-YP614485BNV
CSB-EP614485BNV
CSB-BP614485BNV
CSB-MP614485BNV
CSB-EP614485BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 30S ribosomal protein S13 (rpsM) CSB-YP614486BNV
CSB-EP614486BNV
CSB-BP614486BNV
CSB-MP614486BNV
CSB-EP614486BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Peptide chain release factor 1 (prfA) CSB-YP614487BNV
CSB-EP614487BNV
CSB-BP614487BNV
CSB-MP614487BNV
CSB-EP614487BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP614488BNV
CSB-EP614488BNV
CSB-BP614488BNV
CSB-MP614488BNV
CSB-EP614488BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UPF0289 protein Bxeno_A3895 (Bxeno_A3895) CSB-YP614489BNV
CSB-EP614489BNV
CSB-BP614489BNV
CSB-MP614489BNV
CSB-EP614489BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Putative iron-sulfur cluster insertion protein ErpA 1 (erpA1) CSB-YP614490BNV
CSB-EP614490BNV
CSB-BP614490BNV
CSB-MP614490BNV
CSB-EP614490BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP614491BNV
CSB-EP614491BNV
CSB-BP614491BNV
CSB-MP614491BNV
CSB-EP614491BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UPF0234 protein Bxeno_A3642 (Bxeno_A3642) CSB-YP614492BNV
CSB-EP614492BNV
CSB-BP614492BNV
CSB-MP614492BNV
CSB-EP614492BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 50S ribosomal protein L28 (rpmB) CSB-YP614493BNV
CSB-EP614493BNV
CSB-BP614493BNV
CSB-MP614493BNV
CSB-EP614493BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Disulfide bond formation protein B (dsbB), partial CSB-YP614494BNV1
CSB-EP614494BNV1
CSB-BP614494BNV1
CSB-MP614494BNV1
CSB-EP614494BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans D-amino acid dehydrogenase small subunit (dadA) CSB-YP614495BNV
CSB-EP614495BNV
CSB-BP614495BNV
CSB-MP614495BNV
CSB-EP614495BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 2-keto-4-pentenoate hydratase (mhpD) CSB-YP614496BNV
CSB-EP614496BNV
CSB-BP614496BNV
CSB-MP614496BNV
CSB-EP614496BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP614497BNV
CSB-EP614497BNV
CSB-BP614497BNV
CSB-MP614497BNV
CSB-EP614497BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 50S ribosomal protein L20 (rplT) CSB-YP614498BNV
CSB-EP614498BNV
CSB-BP614498BNV
CSB-MP614498BNV
CSB-EP614498BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UPF0391 membrane protein Bxeno_A2958 (Bxeno_A2958), partial CSB-YP614499BNV
CSB-EP614499BNV
CSB-BP614499BNV
CSB-MP614499BNV
CSB-EP614499BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Protein hfq (hfq) CSB-YP614500BNV
CSB-EP614500BNV
CSB-BP614500BNV
CSB-MP614500BNV
CSB-EP614500BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans DNA ligase (ligA), partial CSB-YP614501BNV
CSB-EP614501BNV
CSB-BP614501BNV
CSB-MP614501BNV
CSB-EP614501BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Lipid-A-disaccharide synthase (lpxB) CSB-YP614502BNV
CSB-EP614502BNV
CSB-BP614502BNV
CSB-MP614502BNV
CSB-EP614502BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP614503BNV
CSB-EP614503BNV
CSB-BP614503BNV
CSB-MP614503BNV
CSB-EP614503BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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