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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cenocepacia ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP615311BAAG
CSB-EP615311BAAG
CSB-BP615311BAAG
CSB-MP615311BAAG
CSB-EP615311BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Large-conductance mechanosensitive channel (mscL), partial CSB-YP615312BAAG1
CSB-EP615312BAAG1
CSB-BP615312BAAG1
CSB-MP615312BAAG1
CSB-EP615312BAAG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Ribonuclease HII (rnhB) CSB-YP615313BAAG
CSB-EP615313BAAG
CSB-BP615313BAAG
CSB-MP615313BAAG
CSB-EP615313BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP615314BAAG
CSB-EP615314BAAG
CSB-BP615314BAAG
CSB-MP615314BAAG
CSB-EP615314BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S2 (rpsB) CSB-YP615315BAAG
CSB-EP615315BAAG
CSB-BP615315BAAG
CSB-MP615315BAAG
CSB-EP615315BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Cysteine--tRNA ligase (cysS) CSB-YP615316BAAG
CSB-EP615316BAAG
CSB-BP615316BAAG
CSB-MP615316BAAG
CSB-EP615316BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Lysine--tRNA ligase (lysS), partial CSB-YP615317BAAG
CSB-EP615317BAAG
CSB-BP615317BAAG
CSB-MP615317BAAG
CSB-EP615317BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S21 2 (rpsU2) CSB-YP615318BAAG
CSB-EP615318BAAG
CSB-BP615318BAAG
CSB-MP615318BAAG
CSB-EP615318BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Argininosuccinate synthase (argG) CSB-YP615319BAAG
CSB-EP615319BAAG
CSB-BP615319BAAG
CSB-MP615319BAAG
CSB-EP615319BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP615320BAAG
CSB-EP615320BAAG
CSB-BP615320BAAG
CSB-MP615320BAAG
CSB-EP615320BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Chemotaxis response regulator protein-glutamate methylesterase 2 (cheB2) CSB-YP615321BAAG
CSB-EP615321BAAG
CSB-BP615321BAAG
CSB-MP615321BAAG
CSB-EP615321BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Catalase-peroxidase 2 (katG2), partial CSB-YP615322BAAG
CSB-EP615322BAAG
CSB-BP615322BAAG
CSB-MP615322BAAG
CSB-EP615322BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia ATP synthase subunit alpha (atpA), partial CSB-YP615323BAAG
CSB-EP615323BAAG
CSB-BP615323BAAG
CSB-MP615323BAAG
CSB-EP615323BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP615324BAAG
CSB-EP615324BAAG
CSB-BP615324BAAG
CSB-MP615324BAAG
CSB-EP615324BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S12 (rpsL) CSB-YP615325BAAG
CSB-EP615325BAAG
CSB-BP615325BAAG
CSB-MP615325BAAG
CSB-EP615325BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 50S ribosomal protein L3 (rplC) CSB-YP615326BAAG
CSB-EP615326BAAG
CSB-BP615326BAAG
CSB-MP615326BAAG
CSB-EP615326BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 50S ribosomal protein L14 (rplN) CSB-YP615327BAAG
CSB-EP615327BAAG
CSB-BP615327BAAG
CSB-MP615327BAAG
CSB-EP615327BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 50S ribosomal protein L15 (rplO) CSB-YP615328BAAG
CSB-EP615328BAAG
CSB-BP615328BAAG
CSB-MP615328BAAG
CSB-EP615328BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP615329BAAG
CSB-EP615329BAAG
CSB-BP615329BAAG
CSB-MP615329BAAG
CSB-EP615329BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP615330BAAG
CSB-EP615330BAAG
CSB-BP615330BAAG
CSB-MP615330BAAG
CSB-EP615330BAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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