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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Keratin, type II cuticular Hb1 (KRT81) CSB-YP618893HU
CSB-EP618893HU
CSB-BP618893HU
CSB-MP618893HU
CSB-EP618893HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA replication licensing factor MCM6 (MCM6), partial CSB-YP618894HU
CSB-EP618894HU
CSB-BP618894HU
CSB-MP618894HU
CSB-EP618894HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Chaperone protein DnaK (dnaK), partial CSB-YP618896BNV
CSB-EP618896BNV
CSB-BP618896BNV
CSB-MP618896BNV
CSB-EP618896BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Heat-inducible transcription repressor HrcA (hrcA) CSB-YP618897BNV
CSB-EP618897BNV
CSB-BP618897BNV
CSB-MP618897BNV
CSB-EP618897BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans LPS-assembly protein lptD (lptD), partial CSB-YP618898BNV
CSB-EP618898BNV
CSB-BP618898BNV
CSB-MP618898BNV
CSB-EP618898BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP618899BNV
CSB-EP618899BNV
CSB-BP618899BNV
CSB-MP618899BNV
CSB-EP618899BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Peptidyl-tRNA hydrolase (pth) CSB-YP618900BNV
CSB-EP618900BNV
CSB-BP618900BNV
CSB-MP618900BNV
CSB-EP618900BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Interleukin-13 receptor subunit alpha-2 (IL13RA2), partial CSB-YP618901HU
CSB-EP618901HU
CSB-BP618901HU
CSB-MP618901HU
CSB-EP618901HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human UPF0378 protein KIAA0100 (KIAA0100), partial CSB-YP618902HU
CSB-EP618902HU
CSB-BP618902HU
CSB-MP618902HU
CSB-EP618902HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human BTB/POZ domain-containing protein KCTD2 (KCTD2) CSB-YP618903HU
CSB-EP618903HU
CSB-BP618903HU
CSB-MP618903HU
CSB-EP618903HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ribosome biogenesis protein BMS1 homolog (BMS1), partial CSB-YP618904HU
CSB-EP618904HU
CSB-BP618904HU
CSB-MP618904HU
CSB-EP618904HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Protein-export protein SecB (secB) CSB-YP618905BNV
CSB-EP618905BNV
CSB-BP618905BNV
CSB-MP618905BNV
CSB-EP618905BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 8-amino-7-oxononanoate synthase (bioF) CSB-YP618906BNV
CSB-EP618906BNV
CSB-BP618906BNV
CSB-MP618906BNV
CSB-EP618906BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Acetylglutamate kinase (argB) CSB-YP618907BNV
CSB-EP618907BNV
CSB-BP618907BNV
CSB-MP618907BNV
CSB-EP618907BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 7-cyano-7-deazaguanine synthase (queC) CSB-YP618908BNV
CSB-EP618908BNV
CSB-BP618908BNV
CSB-MP618908BNV
CSB-EP618908BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein tyrosine phosphatase receptor type C-associated protein (PTPRCAP), partial CSB-YP618909HU1
CSB-EP618909HU1
CSB-BP618909HU1
CSB-MP618909HU1
CSB-EP618909HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Methionyl-tRNA formyltransferase (fmt) CSB-YP618910BNV
CSB-EP618910BNV
CSB-BP618910BNV
CSB-MP618910BNV
CSB-EP618910BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Metabotropic glutamate receptor 7 (GRM7), partial CSB-YP618911HU
CSB-EP618911HU
CSB-BP618911HU
CSB-MP618911HU
CSB-EP618911HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human StAR-related lipid transfer protein 3 (STARD3), partial CSB-YP618912HU1
CSB-EP618912HU1
CSB-BP618912HU1
CSB-MP618912HU1
CSB-EP618912HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein phosphatase 1 regulatory subunit 32 (Ppp1r32) CSB-YP618913MO
CSB-EP618913MO
CSB-BP618913MO
CSB-MP618913MO
CSB-EP618913MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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