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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia xenovorans Probable intracellular septation protein A (Bxeno_A2153), partial CSB-YP621754BNV1
CSB-EP621754BNV1
CSB-BP621754BNV1
CSB-MP621754BNV1
CSB-EP621754BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP621755BNV
CSB-EP621755BNV
CSB-BP621755BNV
CSB-MP621755BNV
CSB-EP621755BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 50S ribosomal protein L31 type B (rpmE2) CSB-YP621756BNV
CSB-EP621756BNV
CSB-BP621756BNV
CSB-MP621756BNV
CSB-EP621756BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Nod factor export ATP-binding protein I (nodI) CSB-YP621757BNV
CSB-EP621757BNV
CSB-BP621757BNV
CSB-MP621757BNV
CSB-EP621757BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Potassium voltage-gated channel subfamily C member 3 (KCNC3), partial CSB-YP621758HU1
CSB-EP621758HU1
CSB-BP621758HU1
CSB-MP621758HU1
CSB-EP621758HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Translation initiation factor IF-2 (infB), partial CSB-YP621759BNV
CSB-EP621759BNV
CSB-BP621759BNV
CSB-MP621759BNV
CSB-EP621759BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ribosome biogenesis protein BOP1 (BOP1) CSB-YP621760HU
CSB-EP621760HU
CSB-BP621760HU
CSB-MP621760HU
CSB-EP621760HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable ATP-dependent RNA helicase DHX34 (DHX34), partial CSB-YP621761HU
CSB-EP621761HU
CSB-BP621761HU
CSB-MP621761HU
CSB-EP621761HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein scribble homolog (SCRIB), partial CSB-YP621762HU
CSB-EP621762HU
CSB-BP621762HU
CSB-MP621762HU
CSB-EP621762HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Single-minded homolog 2 (SIM2) CSB-YP621763HU
CSB-EP621763HU
CSB-BP621763HU
CSB-MP621763HU
CSB-EP621763HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Succinylglutamate desuccinylase (astE) CSB-YP621764BNV
CSB-EP621764BNV
CSB-BP621764BNV
CSB-MP621764BNV
CSB-EP621764BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Imidazolonepropionase (hutI) CSB-YP621765BNV
CSB-EP621765BNV
CSB-BP621765BNV
CSB-MP621765BNV
CSB-EP621765BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein NPAT (NPAT), partial CSB-YP621766HU
CSB-EP621766HU
CSB-BP621766HU
CSB-MP621766HU
CSB-EP621766HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human P-selectin glycoprotein ligand 1 (SELPLG), partial CSB-YP621767HU
CSB-EP621767HU
CSB-BP621767HU
CSB-MP621767HU
CSB-EP621767HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Reticulocalbin-2 (RCN2) CSB-YP621768HU
CSB-EP621768HU
CSB-BP621768HU
CSB-MP621768HU
CSB-EP621768HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP621769BNV
CSB-EP621769BNV
CSB-BP621769BNV
CSB-MP621769BNV
CSB-EP621769BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Potassium-transporting ATPase C chain (kdpC), partial CSB-YP621770BNV
CSB-EP621770BNV
CSB-BP621770BNV
CSB-MP621770BNV
CSB-EP621770BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP621771BNV
CSB-EP621771BNV
CSB-BP621771BNV
CSB-MP621771BNV
CSB-EP621771BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM50A (FAM50A) CSB-YP621772HU
CSB-EP621772HU
CSB-BP621772HU
CSB-MP621772HU
CSB-EP621772HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Bifunctional protein glk (glk), partial CSB-YP621773BNV
CSB-EP621773BNV
CSB-BP621773BNV
CSB-MP621773BNV
CSB-EP621773BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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