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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lawsonia intracellularis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP629864LAAL
CSB-EP629864LAAL
CSB-BP629864LAAL
CSB-MP629864LAAL
CSB-EP629864LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP629865LAAL
CSB-EP629865LAAL
CSB-BP629865LAAL
CSB-MP629865LAAL
CSB-EP629865LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis tRNA modification GTPase MnmE (mnmE) CSB-YP629866LAAL
CSB-EP629866LAAL
CSB-BP629866LAAL
CSB-MP629866LAAL
CSB-EP629866LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP629867LAAL
CSB-EP629867LAAL
CSB-BP629867LAAL
CSB-MP629867LAAL
CSB-EP629867LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP629868LAAL
CSB-EP629868LAAL
CSB-BP629868LAAL
CSB-MP629868LAAL
CSB-EP629868LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L18 (rplR) CSB-YP629869LAAL
CSB-EP629869LAAL
CSB-BP629869LAAL
CSB-MP629869LAAL
CSB-EP629869LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L22 (rplV) CSB-YP629870LAAL
CSB-EP629870LAAL
CSB-BP629870LAAL
CSB-MP629870LAAL
CSB-EP629870LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP629871LAAL
CSB-EP629871LAAL
CSB-BP629871LAAL
CSB-MP629871LAAL
CSB-EP629871LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L7/L12 (rplL) CSB-YP629872LAAL
CSB-EP629872LAAL
CSB-BP629872LAAL
CSB-MP629872LAAL
CSB-EP629872LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Dihydrodipicolinate synthase (dapA) CSB-YP629873LAAL
CSB-EP629873LAAL
CSB-BP629873LAAL
CSB-MP629873LAAL
CSB-EP629873LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP629874LAAL
CSB-EP629874LAAL
CSB-BP629874LAAL
CSB-MP629874LAAL
CSB-EP629874LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP629875LAAL
CSB-EP629875LAAL
CSB-BP629875LAAL
CSB-MP629875LAAL
CSB-EP629875LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP629876LAAL
CSB-EP629876LAAL
CSB-BP629876LAAL
CSB-MP629876LAAL
CSB-EP629876LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L21 (rplU) CSB-YP629877LAAL
CSB-EP629877LAAL
CSB-BP629877LAAL
CSB-MP629877LAAL
CSB-EP629877LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Undecaprenyl-diphosphatase (uppP), partial CSB-YP629878LAAL
CSB-EP629878LAAL
CSB-BP629878LAAL
CSB-MP629878LAAL
CSB-EP629878LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis ATP synthase subunit beta (atpD) CSB-YP629879LAAL
CSB-EP629879LAAL
CSB-BP629879LAAL
CSB-MP629879LAAL
CSB-EP629879LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 30S ribosomal protein S2 (rpsB) CSB-YP629880LAAL
CSB-EP629880LAAL
CSB-BP629880LAAL
CSB-MP629880LAAL
CSB-EP629880LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Thiamine-phosphate synthase (thiE) CSB-YP629881LAAL
CSB-EP629881LAAL
CSB-BP629881LAAL
CSB-MP629881LAAL
CSB-EP629881LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP629882LAAL
CSB-EP629882LAAL
CSB-BP629882LAAL
CSB-MP629882LAAL
CSB-EP629882LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Dihydrodipicolinate reductase (dapB) CSB-YP629883LAAL
CSB-EP629883LAAL
CSB-BP629883LAAL
CSB-MP629883LAAL
CSB-EP629883LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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