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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ralstonia metallidurans Translation initiation factor IF-1 1 (infA1) CSB-YP630784RJG
CSB-EP630784RJG
CSB-BP630784RJG
CSB-MP630784RJG
CSB-EP630784RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP630785RJG
CSB-EP630785RJG
CSB-BP630785RJG
CSB-MP630785RJG
CSB-EP630785RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribosome maturation factor RimP (rimP) CSB-YP630786RJG
CSB-EP630786RJG
CSB-BP630786RJG
CSB-MP630786RJG
CSB-EP630786RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 50S ribosomal protein L9 (rplI) CSB-YP630787RJG
CSB-EP630787RJG
CSB-BP630787RJG
CSB-MP630787RJG
CSB-EP630787RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans UPF0061 protein Rmet_1864 (Rmet_1864), partial CSB-YP630788RJG
CSB-EP630788RJG
CSB-BP630788RJG
CSB-MP630788RJG
CSB-EP630788RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Flagellar transcriptional regulator FlhC (flhC) CSB-YP630789RJG
CSB-EP630789RJG
CSB-BP630789RJG
CSB-MP630789RJG
CSB-EP630789RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP630790RJG
CSB-EP630790RJG
CSB-BP630790RJG
CSB-MP630790RJG
CSB-EP630790RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP630791RJG
CSB-EP630791RJG
CSB-BP630791RJG
CSB-MP630791RJG
CSB-EP630791RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Probable potassium transport system protein kup (kup), partial CSB-YP630792RJG
CSB-EP630792RJG
CSB-BP630792RJG
CSB-MP630792RJG
CSB-EP630792RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP630793RJG
CSB-EP630793RJG
CSB-BP630793RJG
CSB-MP630793RJG
CSB-EP630793RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Chaperone protein hscA homolog (hscA), partial CSB-YP630794RJG
CSB-EP630794RJG
CSB-BP630794RJG
CSB-MP630794RJG
CSB-EP630794RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Urease subunit beta (ureB) CSB-YP630795RJG
CSB-EP630795RJG
CSB-BP630795RJG
CSB-MP630795RJG
CSB-EP630795RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP630796RJG
CSB-EP630796RJG
CSB-BP630796RJG
CSB-MP630796RJG
CSB-EP630796RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Adenine deaminase (Rmet_0890) CSB-YP630797RJG
CSB-EP630797RJG
CSB-BP630797RJG
CSB-MP630797RJG
CSB-EP630797RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Glutamine--tRNA ligase (glnS), partial CSB-YP630798RJG
CSB-EP630798RJG
CSB-BP630798RJG
CSB-MP630798RJG
CSB-EP630798RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribosome maturation factor RimM (rimM) CSB-YP630799RJG
CSB-EP630799RJG
CSB-BP630799RJG
CSB-MP630799RJG
CSB-EP630799RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP630800RJG
CSB-EP630800RJG
CSB-BP630800RJG
CSB-MP630800RJG
CSB-EP630800RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP630801RJG
CSB-EP630801RJG
CSB-BP630801RJG
CSB-MP630801RJG
CSB-EP630801RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP630802RJG
CSB-EP630802RJG
CSB-BP630802RJG
CSB-MP630802RJG
CSB-EP630802RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Dihydroorotase (pyrC) CSB-YP630803RJG
CSB-EP630803RJG
CSB-BP630803RJG
CSB-MP630803RJG
CSB-EP630803RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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