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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizobium leguminosarum bv. viciae N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP630904RKS
CSB-EP630904RKS
CSB-BP630904RKS
CSB-MP630904RKS
CSB-EP630904RKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis ATP synthase gamma chain (atpG) CSB-YP630905LAAL
CSB-EP630905LAAL
CSB-BP630905LAAL
CSB-MP630905LAAL
CSB-EP630905LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP630906LAAL
CSB-EP630906LAAL
CSB-BP630906LAAL
CSB-MP630906LAAL
CSB-EP630906LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Membrane protein insertase YidC (yidC), partial CSB-YP630907LAAL1
CSB-EP630907LAAL1
CSB-BP630907LAAL1
CSB-MP630907LAAL1
CSB-EP630907LAAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Porphobilinogen deaminase (hemC) CSB-YP630908LAAL
CSB-EP630908LAAL
CSB-BP630908LAAL
CSB-MP630908LAAL
CSB-EP630908LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP630909LAAL
CSB-EP630909LAAL
CSB-BP630909LAAL
CSB-MP630909LAAL
CSB-EP630909LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 30S ribosomal protein S8 (rpsH) CSB-YP630910LAAL
CSB-EP630910LAAL
CSB-BP630910LAAL
CSB-MP630910LAAL
CSB-EP630910LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 30S ribosomal protein S19 (rpsS) CSB-YP630911LAAL
CSB-EP630911LAAL
CSB-BP630911LAAL
CSB-MP630911LAAL
CSB-EP630911LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 30S ribosomal protein S7 (rpsG) CSB-YP630912LAAL
CSB-EP630912LAAL
CSB-BP630912LAAL
CSB-MP630912LAAL
CSB-EP630912LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Queuine tRNA-ribosyltransferase (tgt) CSB-YP630913LAAL
CSB-EP630913LAAL
CSB-BP630913LAAL
CSB-MP630913LAAL
CSB-EP630913LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP630914LAAL
CSB-EP630914LAAL
CSB-BP630914LAAL
CSB-MP630914LAAL
CSB-EP630914LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP630915LAAL
CSB-EP630915LAAL
CSB-BP630915LAAL
CSB-MP630915LAAL
CSB-EP630915LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 7-cyano-7-deazaguanine synthase (queC) CSB-YP630916LAAL
CSB-EP630916LAAL
CSB-BP630916LAAL
CSB-MP630916LAAL
CSB-EP630916LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Asparagine--tRNA ligase (asnS) CSB-YP630917LAAL
CSB-EP630917LAAL
CSB-BP630917LAAL
CSB-MP630917LAAL
CSB-EP630917LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Protein translocase subunit SecA (secA), partial CSB-YP630918LAAL
CSB-EP630918LAAL
CSB-BP630918LAAL
CSB-MP630918LAAL
CSB-EP630918LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP630919LAAL
CSB-EP630919LAAL
CSB-BP630919LAAL
CSB-MP630919LAAL
CSB-EP630919LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP630920LAAL
CSB-EP630920LAAL
CSB-BP630920LAAL
CSB-MP630920LAAL
CSB-EP630920LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP630921LAAL
CSB-EP630921LAAL
CSB-BP630921LAAL
CSB-MP630921LAAL
CSB-EP630921LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis ATP synthase subunit delta (atpH) CSB-YP630922LAAL
CSB-EP630922LAAL
CSB-BP630922LAAL
CSB-MP630922LAAL
CSB-EP630922LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP630923LAAL
CSB-EP630923LAAL
CSB-BP630923LAAL
CSB-MP630923LAAL
CSB-EP630923LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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