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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium leguminosarum bv. viciae ATP-dependent protease subunit HslV (hslV) CSB-YP633184RKS
CSB-EP633184RKS
CSB-BP633184RKS
CSB-MP633184RKS
CSB-EP633184RKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. viciae Protein-export protein SecB (secB) CSB-YP633185RKS
CSB-EP633185RKS
CSB-BP633185RKS
CSB-MP633185RKS
CSB-EP633185RKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP633186LAAL
CSB-EP633186LAAL
CSB-BP633186LAAL
CSB-MP633186LAAL
CSB-EP633186LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP633187LAAL
CSB-EP633187LAAL
CSB-BP633187LAAL
CSB-MP633187LAAL
CSB-EP633187LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis tRNA pseudouridine synthase A (truA) CSB-YP633188LAAL
CSB-EP633188LAAL
CSB-BP633188LAAL
CSB-MP633188LAAL
CSB-EP633188LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP633189LAAL
CSB-EP633189LAAL
CSB-BP633189LAAL
CSB-MP633189LAAL
CSB-EP633189LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 30S ribosomal protein S4 (rpsD) CSB-YP633190LAAL
CSB-EP633190LAAL
CSB-BP633190LAAL
CSB-MP633190LAAL
CSB-EP633190LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L5 (rplE) CSB-YP633191LAAL
CSB-EP633191LAAL
CSB-BP633191LAAL
CSB-MP633191LAAL
CSB-EP633191LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L2 (rplB) CSB-YP633192LAAL
CSB-EP633192LAAL
CSB-BP633192LAAL
CSB-MP633192LAAL
CSB-EP633192LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 30S ribosomal protein S12 (rpsL) CSB-YP633193LAAL
CSB-EP633193LAAL
CSB-BP633193LAAL
CSB-MP633193LAAL
CSB-EP633193LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP633194LAAL
CSB-EP633194LAAL
CSB-BP633194LAAL
CSB-MP633194LAAL
CSB-EP633194LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis UPF0173 metal-dependent hydrolase LI0883 (LI0883) CSB-YP633195LAAL
CSB-EP633195LAAL
CSB-BP633195LAAL
CSB-MP633195LAAL
CSB-EP633195LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L28 (rpmB) CSB-YP633196LAAL
CSB-EP633196LAAL
CSB-BP633196LAAL
CSB-MP633196LAAL
CSB-EP633196LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP633197LAAL
CSB-EP633197LAAL
CSB-BP633197LAAL
CSB-MP633197LAAL
CSB-EP633197LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L25 (rplY) CSB-YP633198LAAL
CSB-EP633198LAAL
CSB-BP633198LAAL
CSB-MP633198LAAL
CSB-EP633198LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP633199LAAL
CSB-EP633199LAAL
CSB-BP633199LAAL
CSB-MP633199LAAL
CSB-EP633199LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis GTPase obg (obg) CSB-YP633200LAAL
CSB-EP633200LAAL
CSB-BP633200LAAL
CSB-MP633200LAAL
CSB-EP633200LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Translation initiation factor IF-2 (infB), partial CSB-YP633201LAAL
CSB-EP633201LAAL
CSB-BP633201LAAL
CSB-MP633201LAAL
CSB-EP633201LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Bifunctional enzyme IspD/IspF (ispDF) CSB-YP633202LAAL
CSB-EP633202LAAL
CSB-BP633202LAAL
CSB-MP633202LAAL
CSB-EP633202LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis ATP synthase subunit alpha (atpA), partial CSB-YP633203LAAL
CSB-EP633203LAAL
CSB-BP633203LAAL
CSB-MP633203LAAL
CSB-EP633203LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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