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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus salivarius Segregation and condensation protein A (scpA) CSB-YP635237LAAM
CSB-EP635237LAAM
CSB-BP635237LAAM
CSB-MP635237LAAM
CSB-EP635237LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius DNA repair protein recO (recO) CSB-YP635238LAAM
CSB-EP635238LAAM
CSB-BP635238LAAM
CSB-MP635238LAAM
CSB-EP635238LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Diaminopimelate epimerase (dapF) CSB-YP635239LAAM
CSB-EP635239LAAM
CSB-BP635239LAAM
CSB-MP635239LAAM
CSB-EP635239LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Bifunctional protein pyrR (pyrR) CSB-YP635240LAAM
CSB-EP635240LAAM
CSB-BP635240LAAM
CSB-MP635240LAAM
CSB-EP635240LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius UPF0346 protein LSL_0716 (LSL_0716) CSB-YP635241LAAM
CSB-EP635241LAAM
CSB-BP635241LAAM
CSB-MP635241LAAM
CSB-EP635241LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Arginine--tRNA ligase (argS), partial CSB-YP635242LAAM
CSB-EP635242LAAM
CSB-BP635242LAAM
CSB-MP635242LAAM
CSB-EP635242LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP635243LAAM
CSB-EP635243LAAM
CSB-BP635243LAAM
CSB-MP635243LAAM
CSB-EP635243LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Ribosome maturation factor RimM (rimM) CSB-YP635244LAAM
CSB-EP635244LAAM
CSB-BP635244LAAM
CSB-MP635244LAAM
CSB-EP635244LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Guanylate kinase (gmk) CSB-YP635245LAAM
CSB-EP635245LAAM
CSB-BP635245LAAM
CSB-MP635245LAAM
CSB-EP635245LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Peptide chain release factor 1 (prfA) CSB-YP635246LAAM
CSB-EP635246LAAM
CSB-BP635246LAAM
CSB-MP635246LAAM
CSB-EP635246LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Ribosome maturation factor RimP (rimP) CSB-YP635247LAAM
CSB-EP635247LAAM
CSB-BP635247LAAM
CSB-MP635247LAAM
CSB-EP635247LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius UPF0154 protein LSL_0542 (LSL_0542), partial CSB-YP635248LAAM
CSB-EP635248LAAM
CSB-BP635248LAAM
CSB-MP635248LAAM
CSB-EP635248LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius 50S ribosomal protein L32 (rpmF) CSB-YP635249LAAM
CSB-EP635249LAAM
CSB-BP635249LAAM
CSB-MP635249LAAM
CSB-EP635249LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP635250LAAM
CSB-EP635250LAAM
CSB-BP635250LAAM
CSB-MP635250LAAM
CSB-EP635250LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Leucine--tRNA ligase (leuS), partial CSB-YP635251LAAM
CSB-EP635251LAAM
CSB-BP635251LAAM
CSB-MP635251LAAM
CSB-EP635251LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Peptide chain release factor 3 (prfC), partial CSB-YP635252LAAM
CSB-EP635252LAAM
CSB-BP635252LAAM
CSB-MP635252LAAM
CSB-EP635252LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius UPF0173 metal-dependent hydrolase LSL_0324 (LSL_0324) CSB-YP635253LAAM
CSB-EP635253LAAM
CSB-BP635253LAAM
CSB-MP635253LAAM
CSB-EP635253LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Elongation factor G (fusA), partial CSB-YP635254LAAM
CSB-EP635254LAAM
CSB-BP635254LAAM
CSB-MP635254LAAM
CSB-EP635254LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius Serine--tRNA ligase (serS) CSB-YP635255LAAM
CSB-EP635255LAAM
CSB-BP635255LAAM
CSB-MP635255LAAM
CSB-EP635255LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus salivarius DNA replication and repair protein recF (recF) CSB-YP635256LAAM
CSB-EP635256LAAM
CSB-BP635256LAAM
CSB-MP635256LAAM
CSB-EP635256LAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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