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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia metallidurans UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP635778RJG
CSB-EP635778RJG
CSB-BP635778RJG
CSB-MP635778RJG
CSB-EP635778RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 50S ribosomal protein L27 (rpmA) CSB-YP635779RJG
CSB-EP635779RJG
CSB-BP635779RJG
CSB-MP635779RJG
CSB-EP635779RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Phosphonates import ATP-binding protein PhnC 2 (phnC2) CSB-YP635780RJG
CSB-EP635780RJG
CSB-BP635780RJG
CSB-MP635780RJG
CSB-EP635780RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP635781RJG
CSB-EP635781RJG
CSB-BP635781RJG
CSB-MP635781RJG
CSB-EP635781RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans UPF0234 protein Rmet_2899 (Rmet_2899) CSB-YP635782RJG
CSB-EP635782RJG
CSB-BP635782RJG
CSB-MP635782RJG
CSB-EP635782RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Pantothenate synthetase (panC) CSB-YP635783RJG
CSB-EP635783RJG
CSB-BP635783RJG
CSB-MP635783RJG
CSB-EP635783RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Protein TolB (tolB) CSB-YP635784RJG
CSB-EP635784RJG
CSB-BP635784RJG
CSB-MP635784RJG
CSB-EP635784RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 30S ribosomal protein S21 2 (rpsU2) CSB-YP635785RJG
CSB-EP635785RJG
CSB-BP635785RJG
CSB-MP635785RJG
CSB-EP635785RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Nod factor export ATP-binding protein I (nodI) CSB-YP635786RJG
CSB-EP635786RJG
CSB-BP635786RJG
CSB-MP635786RJG
CSB-EP635786RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Elongation factor 4 (lepA), partial CSB-YP635787RJG
CSB-EP635787RJG
CSB-BP635787RJG
CSB-MP635787RJG
CSB-EP635787RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Acetyl-coenzyme A synthetase (acsA), partial CSB-YP635788RJG
CSB-EP635788RJG
CSB-BP635788RJG
CSB-MP635788RJG
CSB-EP635788RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Glutamate--tRNA ligase (gltX) CSB-YP635789RJG
CSB-EP635789RJG
CSB-BP635789RJG
CSB-MP635789RJG
CSB-EP635789RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Nucleoside diphosphate kinase (ndk) CSB-YP635790RJG
CSB-EP635790RJG
CSB-BP635790RJG
CSB-MP635790RJG
CSB-EP635790RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Probable Fe (2+)-trafficking protein (Rmet_2071) CSB-YP635791RJG
CSB-EP635791RJG
CSB-BP635791RJG
CSB-MP635791RJG
CSB-EP635791RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 30S ribosomal protein S18 (rpsR) CSB-YP635792RJG
CSB-EP635792RJG
CSB-BP635792RJG
CSB-MP635792RJG
CSB-EP635792RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP635793RJG
CSB-EP635793RJG
CSB-BP635793RJG
CSB-MP635793RJG
CSB-EP635793RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Putative phosphotransferase Rmet_1452 (Rmet_1452) CSB-YP635794RJG
CSB-EP635794RJG
CSB-BP635794RJG
CSB-MP635794RJG
CSB-EP635794RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP635795RJG
CSB-EP635795RJG
CSB-BP635795RJG
CSB-MP635795RJG
CSB-EP635795RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans [Protein-PII] uridylyltransferase (glnD), partial CSB-YP635796RJG
CSB-EP635796RJG
CSB-BP635796RJG
CSB-MP635796RJG
CSB-EP635796RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 4-hydroxy-2-oxovalerate aldolase 1 (Rmet_1319) CSB-YP635797RJG
CSB-EP635797RJG
CSB-BP635797RJG
CSB-MP635797RJG
CSB-EP635797RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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