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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Rhizobium leguminosarum bv. viciae Translation initiation factor IF-2 (infB), partial CSB-YP638122RKS
CSB-EP638122RKS
CSB-BP638122RKS
CSB-MP638122RKS
CSB-EP638122RKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. viciae Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP638123RKS
CSB-EP638123RKS
CSB-BP638123RKS
CSB-MP638123RKS
CSB-EP638123RKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. viciae Maf-like protein RL0002 (RL0002) CSB-YP638124RKS
CSB-EP638124RKS
CSB-BP638124RKS
CSB-MP638124RKS
CSB-EP638124RKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Alanine--tRNA ligase (alaS), partial CSB-YP638125LAAL
CSB-EP638125LAAL
CSB-BP638125LAAL
CSB-MP638125LAAL
CSB-EP638125LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP638126LAAL
CSB-EP638126LAAL
CSB-BP638126LAAL
CSB-MP638126LAAL
CSB-EP638126LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis ATP synthase subunit c (atpE), partial CSB-YP638127LAAL1
CSB-EP638127LAAL1
CSB-BP638127LAAL1
CSB-MP638127LAAL1
CSB-EP638127LAAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP638128LAAL
CSB-EP638128LAAL
CSB-BP638128LAAL
CSB-MP638128LAAL
CSB-EP638128LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 30S ribosomal protein S11 (rpsK) CSB-YP638129LAAL
CSB-EP638129LAAL
CSB-BP638129LAAL
CSB-MP638129LAAL
CSB-EP638129LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L24 (rplX) CSB-YP638130LAAL
CSB-EP638130LAAL
CSB-BP638130LAAL
CSB-MP638130LAAL
CSB-EP638130LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L23 (rplW) CSB-YP638131LAAL
CSB-EP638131LAAL
CSB-BP638131LAAL
CSB-MP638131LAAL
CSB-EP638131LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Elongation factor Tu (tuf) CSB-YP638132LAAL
CSB-EP638132LAAL
CSB-BP638132LAAL
CSB-MP638132LAAL
CSB-EP638132LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Cytidylate kinase (cmk) CSB-YP638133LAAL
CSB-EP638133LAAL
CSB-BP638133LAAL
CSB-MP638133LAAL
CSB-EP638133LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Sugar fermentation stimulation protein homolog (sfsA) CSB-YP638134LAAL
CSB-EP638134LAAL
CSB-BP638134LAAL
CSB-MP638134LAAL
CSB-EP638134LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP638135LAAL
CSB-EP638135LAAL
CSB-BP638135LAAL
CSB-MP638135LAAL
CSB-EP638135LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Trigger factor (tig) CSB-YP638136LAAL
CSB-EP638136LAAL
CSB-BP638136LAAL
CSB-MP638136LAAL
CSB-EP638136LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 5'-nucleotidase surE (surE) CSB-YP638137LAAL
CSB-EP638137LAAL
CSB-BP638137LAAL
CSB-MP638137LAAL
CSB-EP638137LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis D-alanine--D-alanine ligase (ddl) CSB-YP638138LAAL
CSB-EP638138LAAL
CSB-BP638138LAAL
CSB-MP638138LAAL
CSB-EP638138LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 10 kDa chaperonin (groS) CSB-YP638139LAAL
CSB-EP638139LAAL
CSB-BP638139LAAL
CSB-MP638139LAAL
CSB-EP638139LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L13 (rplM) CSB-YP638140LAAL
CSB-EP638140LAAL
CSB-BP638140LAAL
CSB-MP638140LAAL
CSB-EP638140LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis UPF0042 nucleotide-binding protein LI0459 (LI0459) CSB-YP638141LAAL
CSB-EP638141LAAL
CSB-BP638141LAAL
CSB-MP638141LAAL
CSB-EP638141LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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