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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium etli Putative ribose/galactose/methyl galactoside import ATP-binding protein 2 (RHE_CH03989), partial CSB-YP646474RKK
CSB-EP646474RKK
CSB-BP646474RKK
CSB-MP646474RKK
CSB-EP646474RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0314 protein RHE_CH03951 (RHE_CH03951), partial CSB-YP646475RKK1
CSB-EP646475RKK1
CSB-BP646475RKK1
CSB-MP646475RKK1
CSB-EP646475RKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli ATP synthase subunit delta (atpH) CSB-YP646476RKK
CSB-EP646476RKK
CSB-BP646476RKK
CSB-MP646476RKK
CSB-EP646476RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 50S ribosomal protein L28 (rpmB) CSB-YP646477RKK
CSB-EP646477RKK
CSB-BP646477RKK
CSB-MP646477RKK
CSB-EP646477RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli NADH-quinone oxidoreductase subunit I 2 (nuoI2) CSB-YP646478RKK
CSB-EP646478RKK
CSB-BP646478RKK
CSB-MP646478RKK
CSB-EP646478RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Glutamate--tRNA ligase (gltX) CSB-YP646479RKK
CSB-EP646479RKK
CSB-BP646479RKK
CSB-MP646479RKK
CSB-EP646479RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0335 protein RHE_CH03546 (RHE_CH03546) CSB-YP646480RKK
CSB-EP646480RKK
CSB-BP646480RKK
CSB-MP646480RKK
CSB-EP646480RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Adenine deaminase 2 (ade2), partial CSB-YP646481RKK
CSB-EP646481RKK
CSB-BP646481RKK
CSB-MP646481RKK
CSB-EP646481RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Urease subunit beta (ureB) CSB-YP646482RKK
CSB-EP646482RKK
CSB-BP646482RKK
CSB-MP646482RKK
CSB-EP646482RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Adenine deaminase 1 (ade1), partial CSB-YP646483RKK
CSB-EP646483RKK
CSB-BP646483RKK
CSB-MP646483RKK
CSB-EP646483RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli C4-dicarboxylate transport protein (dctA), partial CSB-YP646484RKK
CSB-EP646484RKK
CSB-BP646484RKK
CSB-MP646484RKK
CSB-EP646484RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP646485RKK
CSB-EP646485RKK
CSB-BP646485RKK
CSB-MP646485RKK
CSB-EP646485RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Probable potassium transport system protein kup 2 (kup2), partial CSB-YP646486RKK
CSB-EP646486RKK
CSB-BP646486RKK
CSB-MP646486RKK
CSB-EP646486RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0283 membrane protein RHE_CH02332 (RHE_CH02332), partial CSB-YP646487RKK1
CSB-EP646487RKK1
CSB-BP646487RKK1
CSB-MP646487RKK1
CSB-EP646487RKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP646488RKK
CSB-EP646488RKK
CSB-BP646488RKK
CSB-MP646488RKK
CSB-EP646488RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Threonine--tRNA ligase (thrS), partial CSB-YP646489RKK
CSB-EP646489RKK
CSB-BP646489RKK
CSB-MP646489RKK
CSB-EP646489RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phosphopantetheine adenylyltransferase (coaD) CSB-YP646490RKK
CSB-EP646490RKK
CSB-BP646490RKK
CSB-MP646490RKK
CSB-EP646490RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP646491RKK
CSB-EP646491RKK
CSB-BP646491RKK
CSB-MP646491RKK
CSB-EP646491RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP646492RKK
CSB-EP646492RKK
CSB-BP646492RKK
CSB-MP646492RKK
CSB-EP646492RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 5'-nucleotidase surE (surE) CSB-YP646493RKK
CSB-EP646493RKK
CSB-BP646493RKK
CSB-MP646493RKK
CSB-EP646493RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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