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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella dysenteriae serotype 1 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (otsA) CSB-YP656929SGF
CSB-EP656929SGF
CSB-BP656929SGF
CSB-MP656929SGF
CSB-EP656929SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP656930SGF
CSB-EP656930SGF
CSB-BP656930SGF
CSB-MP656930SGF
CSB-EP656930SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UvrABC system protein C (uvrC), partial CSB-YP656931SGF
CSB-EP656931SGF
CSB-BP656931SGF
CSB-MP656931SGF
CSB-EP656931SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Pyrimidine monooxygenase RutA (rutA) CSB-YP656932SGF
CSB-EP656932SGF
CSB-BP656932SGF
CSB-MP656932SGF
CSB-EP656932SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP656933SGF
CSB-EP656933SGF
CSB-BP656933SGF
CSB-MP656933SGF
CSB-EP656933SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Elongation factor P-like protein (yeiP) CSB-YP656934SGF
CSB-EP656934SGF
CSB-BP656934SGF
CSB-MP656934SGF
CSB-EP656934SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Biotin synthase (bioB) CSB-YP656935SGF
CSB-EP656935SGF
CSB-BP656935SGF
CSB-MP656935SGF
CSB-EP656935SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 DNA protection during starvation protein (dps) CSB-YP656936SGF
CSB-EP656936SGF
CSB-BP656936SGF
CSB-MP656936SGF
CSB-EP656936SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Molybdenum import ATP-binding protein ModC (modC) CSB-YP656937SGF
CSB-EP656937SGF
CSB-BP656937SGF
CSB-MP656937SGF
CSB-EP656937SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0271 protein YbgL (ybgL) CSB-YP656938SGF
CSB-EP656938SGF
CSB-BP656938SGF
CSB-MP656938SGF
CSB-EP656938SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Leucine--tRNA ligase (leuS), partial CSB-YP656939SGF
CSB-EP656939SGF
CSB-BP656939SGF
CSB-MP656939SGF
CSB-EP656939SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Enterobactin exporter EntS (entS), partial CSB-YP656940SGF
CSB-EP656940SGF
CSB-BP656940SGF
CSB-MP656940SGF
CSB-EP656940SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Gamma-glutamyl phosphate reductase (proA) CSB-YP656941SGF
CSB-EP656941SGF
CSB-BP656941SGF
CSB-MP656941SGF
CSB-EP656941SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 tRNA 2-selenouridine synthase (selU) CSB-YP656942SGF
CSB-EP656942SGF
CSB-BP656942SGF
CSB-MP656942SGF
CSB-EP656942SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0345 protein YaiE (yaiE) CSB-YP656943SGF
CSB-EP656943SGF
CSB-BP656943SGF
CSB-MP656943SGF
CSB-EP656943SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP656944SGF
CSB-EP656944SGF
CSB-BP656944SGF
CSB-MP656944SGF
CSB-EP656944SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 7-cyano-7-deazaguanine synthase (queC) CSB-YP656945SGF
CSB-EP656945SGF
CSB-BP656945SGF
CSB-MP656945SGF
CSB-EP656945SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0294 protein yafD (yafD) CSB-YP656946SGF
CSB-EP656946SGF
CSB-BP656946SGF
CSB-MP656946SGF
CSB-EP656946SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP656947SGF
CSB-EP656947SGF
CSB-BP656947SGF
CSB-MP656947SGF
CSB-EP656947SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 [Protein-PII] uridylyltransferase (glnD), partial CSB-YP656948SGF
CSB-EP656948SGF
CSB-BP656948SGF
CSB-MP656948SGF
CSB-EP656948SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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