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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfovibrio desulfuricans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP657290DAAF
CSB-EP657290DAAF
CSB-BP657290DAAF
CSB-MP657290DAAF
CSB-EP657290DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans 30S ribosomal protein S12 (rpsL) CSB-YP657291DAAF
CSB-EP657291DAAF
CSB-BP657291DAAF
CSB-MP657291DAAF
CSB-EP657291DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans 30S ribosomal protein S19 (rpsS) CSB-YP657292DAAF
CSB-EP657292DAAF
CSB-BP657292DAAF
CSB-MP657292DAAF
CSB-EP657292DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans 50S ribosomal protein L24 (rplX) CSB-YP657293DAAF
CSB-EP657293DAAF
CSB-BP657293DAAF
CSB-MP657293DAAF
CSB-EP657293DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans 30S ribosomal protein S13 (rpsM) CSB-YP657294DAAF
CSB-EP657294DAAF
CSB-BP657294DAAF
CSB-MP657294DAAF
CSB-EP657294DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP657295DAAF
CSB-EP657295DAAF
CSB-BP657295DAAF
CSB-MP657295DAAF
CSB-EP657295DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Isoleucine--tRNA ligase (ileS), partial CSB-YP657296DAAF
CSB-EP657296DAAF
CSB-BP657296DAAF
CSB-MP657296DAAF
CSB-EP657296DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP657297DAAF
CSB-EP657297DAAF
CSB-BP657297DAAF
CSB-MP657297DAAF
CSB-EP657297DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP657298DAAF
CSB-EP657298DAAF
CSB-BP657298DAAF
CSB-MP657298DAAF
CSB-EP657298DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Acetylglutamate kinase (argB) CSB-YP657299DAAF
CSB-EP657299DAAF
CSB-BP657299DAAF
CSB-MP657299DAAF
CSB-EP657299DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Probable GTP-binding protein EngB (engB) CSB-YP657300DAAF
CSB-EP657300DAAF
CSB-BP657300DAAF
CSB-MP657300DAAF
CSB-EP657300DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Ribonuclease 3 (rnc) CSB-YP657301DAAF
CSB-EP657301DAAF
CSB-BP657301DAAF
CSB-MP657301DAAF
CSB-EP657301DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Gamma-glutamyl phosphate reductase (proA) CSB-YP657302DAAF
CSB-EP657302DAAF
CSB-BP657302DAAF
CSB-MP657302DAAF
CSB-EP657302DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP657303DAAF
CSB-EP657303DAAF
CSB-BP657303DAAF
CSB-MP657303DAAF
CSB-EP657303DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans 30S ribosomal protein S6 (rpsF) CSB-YP657304DAAF
CSB-EP657304DAAF
CSB-BP657304DAAF
CSB-MP657304DAAF
CSB-EP657304DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Uridylate kinase (pyrH) CSB-YP657305DAAF
CSB-EP657305DAAF
CSB-BP657305DAAF
CSB-MP657305DAAF
CSB-EP657305DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP657306DAAF
CSB-EP657306DAAF
CSB-BP657306DAAF
CSB-MP657306DAAF
CSB-EP657306DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP657307DAAF
CSB-EP657307DAAF
CSB-BP657307DAAF
CSB-MP657307DAAF
CSB-EP657307DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP657308DAAF
CSB-EP657308DAAF
CSB-BP657308DAAF
CSB-MP657308DAAF
CSB-EP657308DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans ATP synthase subunit beta (atpD) CSB-YP657309DAAF
CSB-EP657309DAAF
CSB-BP657309DAAF
CSB-MP657309DAAF
CSB-EP657309DAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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