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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella dysenteriae serotype 1 Glutathione transport system permease protein gsiD (gsiD), partial CSB-YP657630SGF1
CSB-EP657630SGF1
CSB-BP657630SGF1
CSB-MP657630SGF1
CSB-EP657630SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Protein TolB (tolB) CSB-YP657631SGF
CSB-EP657631SGF
CSB-BP657631SGF
CSB-MP657631SGF
CSB-EP657631SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Glucosamine-6-phosphate deaminase (nagB) CSB-YP657632SGF
CSB-EP657632SGF
CSB-BP657632SGF
CSB-MP657632SGF
CSB-EP657632SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Octanoyltransferase (lipB) CSB-YP657633SGF
CSB-EP657633SGF
CSB-BP657633SGF
CSB-MP657633SGF
CSB-EP657633SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Esterase FrsA (frsA) CSB-YP657634SGF
CSB-EP657634SGF
CSB-BP657634SGF
CSB-MP657634SGF
CSB-EP657634SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Ferrochelatase (hemH) CSB-YP657635SGF
CSB-EP657635SGF
CSB-BP657635SGF
CSB-MP657635SGF
CSB-EP657635SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0178 protein yaiI (yaiI) CSB-YP657636SGF
CSB-EP657636SGF
CSB-BP657636SGF
CSB-MP657636SGF
CSB-EP657636SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP657637SGF
CSB-EP657637SGF
CSB-BP657637SGF
CSB-MP657637SGF
CSB-EP657637SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Trigger factor (tig) CSB-YP657638SGF
CSB-EP657638SGF
CSB-BP657638SGF
CSB-MP657638SGF
CSB-EP657638SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP657639SGF
CSB-EP657639SGF
CSB-BP657639SGF
CSB-MP657639SGF
CSB-EP657639SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Proline--tRNA ligase (proS), partial CSB-YP657640SGF
CSB-EP657640SGF
CSB-BP657640SGF
CSB-MP657640SGF
CSB-EP657640SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Chaperone protein skp (skp) CSB-YP657641SGF
CSB-EP657641SGF
CSB-BP657641SGF
CSB-MP657641SGF
CSB-EP657641SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0325 protein yaeH (yaeH) CSB-YP657642SGF
CSB-EP657642SGF
CSB-BP657642SGF
CSB-MP657642SGF
CSB-EP657642SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 GMP reductase (guaC) CSB-YP657643SGF
CSB-EP657643SGF
CSB-BP657643SGF
CSB-MP657643SGF
CSB-EP657643SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Cell division protein FtsQ (ftsQ), partial CSB-YP657644SGF
CSB-EP657644SGF
CSB-BP657644SGF
CSB-MP657644SGF
CSB-EP657644SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 2-isopropylmalate synthase (leuA), partial CSB-YP657645SGF
CSB-EP657645SGF
CSB-BP657645SGF
CSB-MP657645SGF
CSB-EP657645SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Chaperone surA (surA) CSB-YP657646SGF
CSB-EP657646SGF
CSB-BP657646SGF
CSB-MP657646SGF
CSB-EP657646SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP657647SGF
CSB-EP657647SGF
CSB-BP657647SGF
CSB-MP657647SGF
CSB-EP657647SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Non-specific ribonucleoside hydrolase rihC (rihC) CSB-YP657648SGF
CSB-EP657648SGF
CSB-BP657648SGF
CSB-MP657648SGF
CSB-EP657648SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Chaperone protein DnaJ (dnaJ) CSB-YP657649SGF
CSB-EP657649SGF
CSB-BP657649SGF
CSB-MP657649SGF
CSB-EP657649SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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