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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Diacylglycerol kinase alpha (DGKA) CSB-EP006832HUc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Dickkopf-related protein 4 (DKK4) CSB-EP883367HUa2 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Chlamydophila psittaci Major outer membrane porin (ompA) CSB-EP669359DSMe3 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Dual specificity mitogen-activated protein kinase kinase 3 (MAP2K3) CSB-EP013412HUh2 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Basic leucine zipper and W2 domain-containing protein 2 (BZW2) CSB-EP896544HUc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme,Biotinylated CSB-EP357414SXPm4-B 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Bordetella pertussis Pertussis toxin subunit 1 (ptxA) CSB-EP356423BUAa6 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Deoxyribonuclease gamma (Dnase1l3) CSB-EP007052MOb0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Lysyl oxidase homolog 1 (LOXL1) CSB-EP013040HU(A4)c7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Delta and Notch-like epidermal growth factor-related receptor (DNER), partial CSB-EP818784HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Escherichia coli DNA adenine methylase (dam) CSB-EP360101ENVh2 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Cyclin-G-associated kinase (GAK), partial CSB-EP009190HUh2 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Rat Serum albumin (Alb) CSB-EP001561RA 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mesocricetus auratus complement receptor type 2 isoform X1 (Cr2), partial CSB-EP4835MRG2 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Troponin T, cardiac muscle (Tnnt2) CSB-EP024016MOi10 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Heterogeneous nuclear ribonucleoproteins A2/B1 (HNRNPA2B1) CSB-EP2576HUa0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Ragulator complex protein LAMTOR5 (LAMTOR5) CSB-EP010161HUe3 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Transcription factor SOX-1 (SOX1) CSB-EP022417HUb1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Lysine-specific demethylase 6B (KDM6B), partial CSB-EP517674HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Chicken Alpha-N-acetylgalactosaminidase (NAGA) CSB-EP856189CH 1mg(1mg*1 or 500ug*2)/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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