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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Putative phosphotransferase BT9727_4034 (BT9727_4034) CSB-YP760731BAAE
CSB-EP760731BAAE
CSB-BP760731BAAE
CSB-MP760731BAAE
CSB-EP760731BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphate import ATP-binding protein PstB (pstB) CSB-YP760732BAAE
CSB-EP760732BAAE
CSB-BP760732BAAE
CSB-MP760732BAAE
CSB-EP760732BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP760733BAAE
CSB-EP760733BAAE
CSB-BP760733BAAE
CSB-MP760733BAAE
CSB-EP760733BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP760734BAAE
CSB-EP760734BAAE
CSB-BP760734BAAE
CSB-MP760734BAAE
CSB-EP760734BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphopentomutase (deoB) CSB-YP760735BAAE
CSB-EP760735BAAE
CSB-BP760735BAAE
CSB-MP760735BAAE
CSB-EP760735BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methylthioribose kinase (mtnK) CSB-YP760736BAAE
CSB-EP760736BAAE
CSB-BP760736BAAE
CSB-MP760736BAAE
CSB-EP760736BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Protoheme IX farnesyltransferase (ctaB), partial CSB-YP760737BAAE
CSB-EP760737BAAE
CSB-BP760737BAAE
CSB-MP760737BAAE
CSB-EP760737BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0747 protein BT9727_3661 (BT9727_3661), partial CSB-YP760738BAAE
CSB-EP760738BAAE
CSB-BP760738BAAE
CSB-MP760738BAAE
CSB-EP760738BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Isoleucine--tRNA ligase 1 (ileS1), partial CSB-YP760739BAAE
CSB-EP760739BAAE
CSB-BP760739BAAE
CSB-MP760739BAAE
CSB-EP760739BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Carbamoyl-phosphate synthase small chain (carA) CSB-YP760740BAAE
CSB-EP760740BAAE
CSB-BP760740BAAE
CSB-MP760740BAAE
CSB-EP760740BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Guanylate kinase (gmk) CSB-YP760741BAAE
CSB-EP760741BAAE
CSB-BP760741BAAE
CSB-MP760741BAAE
CSB-EP760741BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphate acyltransferase (plsX) CSB-YP760742BAAE
CSB-EP760742BAAE
CSB-BP760742BAAE
CSB-MP760742BAAE
CSB-EP760742BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP760743BAAE
CSB-EP760743BAAE
CSB-BP760743BAAE
CSB-MP760743BAAE
CSB-EP760743BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Proline--tRNA ligase 1 (proS1), partial CSB-YP760744BAAE
CSB-EP760744BAAE
CSB-BP760744BAAE
CSB-MP760744BAAE
CSB-EP760744BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB), partial CSB-YP760745BAAE
CSB-EP760745BAAE
CSB-BP760745BAAE
CSB-MP760745BAAE
CSB-EP760745BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0154 protein BT9727_3434 (BT9727_3434), partial CSB-YP760746BAAE
CSB-EP760746BAAE
CSB-BP760746BAAE
CSB-MP760746BAAE
CSB-EP760746BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Imidazolonepropionase (hutI) CSB-YP760747BAAE
CSB-EP760747BAAE
CSB-BP760747BAAE
CSB-MP760747BAAE
CSB-EP760747BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0145 protein BT9727_3206 (BT9727_3206) CSB-YP760748BAAE
CSB-EP760748BAAE
CSB-BP760748BAAE
CSB-MP760748BAAE
CSB-EP760748BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Protein ArsC (arsC) CSB-YP760749BAAE
CSB-EP760749BAAE
CSB-BP760749BAAE
CSB-MP760749BAAE
CSB-EP760749BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glutamate 5-kinase (proB) CSB-YP760750BAAE
CSB-EP760750BAAE
CSB-BP760750BAAE
CSB-MP760750BAAE
CSB-EP760750BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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