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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Histidinol-phosphate aminotransferase 2 (hisC2) CSB-YP760751BAAE
CSB-EP760751BAAE
CSB-BP760751BAAE
CSB-MP760751BAAE
CSB-EP760751BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0397 protein BT9727_2423 (BT9727_2423), partial CSB-YP760752BAAE1
CSB-EP760752BAAE1
CSB-BP760752BAAE1
CSB-MP760752BAAE1
CSB-EP760752BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 5-dehydro-2-deoxygluconokinase (iolC) CSB-YP760753BAAE
CSB-EP760753BAAE
CSB-BP760753BAAE
CSB-MP760753BAAE
CSB-EP760753BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP760754BAAE
CSB-EP760754BAAE
CSB-BP760754BAAE
CSB-MP760754BAAE
CSB-EP760754BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Uncharacterized HTH-type transcriptional regulator BT9727_1775 (BT9727_1775) CSB-YP760755BAAE
CSB-EP760755BAAE
CSB-BP760755BAAE
CSB-MP760755BAAE
CSB-EP760755BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP760756BAAE
CSB-EP760756BAAE
CSB-BP760756BAAE
CSB-MP760756BAAE
CSB-EP760756BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0398 protein BT9727_1438 (BT9727_1438) CSB-YP760757BAAE
CSB-EP760757BAAE
CSB-BP760757BAAE
CSB-MP760757BAAE
CSB-EP760757BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP760758BAAE
CSB-EP760758BAAE
CSB-BP760758BAAE
CSB-MP760758BAAE
CSB-EP760758BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 3-dehydroquinate synthase (aroB) CSB-YP760759BAAE
CSB-EP760759BAAE
CSB-BP760759BAAE
CSB-MP760759BAAE
CSB-EP760759BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian GTPase Der (der) CSB-YP760760BAAE
CSB-EP760760BAAE
CSB-BP760760BAAE
CSB-MP760760BAAE
CSB-EP760760BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Flavohemoprotein (hmp) CSB-YP760761BAAE
CSB-EP760761BAAE
CSB-BP760761BAAE
CSB-MP760761BAAE
CSB-EP760761BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable inorganic polyphosphate/ATP-NAD kinase 1 (ppnK1) CSB-YP760762BAAE
CSB-EP760762BAAE
CSB-BP760762BAAE
CSB-MP760762BAAE
CSB-EP760762BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP760763BAAE
CSB-EP760763BAAE
CSB-BP760763BAAE
CSB-MP760763BAAE
CSB-EP760763BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0145 protein BT9727_0921 (BT9727_0921) CSB-YP760764BAAE
CSB-EP760764BAAE
CSB-BP760764BAAE
CSB-MP760764BAAE
CSB-EP760764BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative 3-methyladenine DNA glycosylase (BT9727_0773) CSB-YP760765BAAE
CSB-EP760765BAAE
CSB-BP760765BAAE
CSB-MP760765BAAE
CSB-EP760765BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Potassium-transporting ATPase C chain (kdpC), partial CSB-YP760766BAAE1
CSB-EP760766BAAE1
CSB-BP760766BAAE1
CSB-MP760766BAAE1
CSB-EP760766BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0229 protein BT9727_0462 (BT9727_0462) CSB-YP760767BAAE
CSB-EP760767BAAE
CSB-BP760767BAAE
CSB-MP760767BAAE
CSB-EP760767BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Regulatory protein recX (recX) CSB-YP760768BAAE
CSB-EP760768BAAE
CSB-BP760768BAAE
CSB-MP760768BAAE
CSB-EP760768BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Thiamine-phosphate synthase (thiE) CSB-YP760769BAAE
CSB-EP760769BAAE
CSB-BP760769BAAE
CSB-MP760769BAAE
CSB-EP760769BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP760770BAAE
CSB-EP760770BAAE
CSB-BP760770BAAE
CSB-MP760770BAAE
CSB-EP760770BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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