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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Sterol 24-C-methyltransferase (ERG6) CSB-YP762233DOT
CSB-EP762233DOT
CSB-BP762233DOT
CSB-MP762233DOT
CSB-EP762233DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nuclear fusion protein KAR5 (KAR5), partial CSB-YP762234DOT1
CSB-EP762234DOT1
CSB-BP762234DOT1
CSB-MP762234DOT1
CSB-EP762234DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA replication complex GINS protein PSF2 (PSF2) CSB-YP762235DOT
CSB-EP762235DOT
CSB-BP762235DOT
CSB-MP762235DOT
CSB-EP762235DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii T-complex protein 1 subunit delta (CCT4), partial CSB-YP762236DOT
CSB-EP762236DOT
CSB-BP762236DOT
CSB-MP762236DOT
CSB-EP762236DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy-related protein 9 (ATG9), partial CSB-YP762237DOT
CSB-EP762237DOT
CSB-BP762237DOT
CSB-MP762237DOT
CSB-EP762237DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Peptidyl-prolyl isomerase CWC27 (CWC27) CSB-YP762238DOT
CSB-EP762238DOT
CSB-BP762238DOT
CSB-MP762238DOT
CSB-EP762238DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mannose-6-phosphate isomerase (PMI1) CSB-YP762239DOT
CSB-EP762239DOT
CSB-BP762239DOT
CSB-MP762239DOT
CSB-EP762239DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytochrome c oxidase assembly protein COX19 (COX19) CSB-YP762240DOT
CSB-EP762240DOT
CSB-BP762240DOT
CSB-MP762240DOT
CSB-EP762240DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondria fission 1 protein (FIS1), partial CSB-YP762241DOT
CSB-EP762241DOT
CSB-BP762241DOT
CSB-MP762241DOT
CSB-EP762241DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal protein L2 (RPL2) CSB-YP762242DOT
CSB-EP762242DOT
CSB-BP762242DOT
CSB-MP762242DOT
CSB-EP762242DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Topoisomerase I damage affected protein 11 (TDA11) CSB-YP762243DOT
CSB-EP762243DOT
CSB-BP762243DOT
CSB-MP762243DOT
CSB-EP762243DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Restriction of telomere capping protein 1 (RTC1), partial CSB-YP762244DOT
CSB-EP762244DOT
CSB-BP762244DOT
CSB-MP762244DOT
CSB-EP762244DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP762245DOT
CSB-EP762245DOT
CSB-BP762245DOT
CSB-MP762245DOT
CSB-EP762245DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein transport protein SEC24 (SEC24), partial CSB-YP762246DOT
CSB-EP762246DOT
CSB-BP762246DOT
CSB-MP762246DOT
CSB-EP762246DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RNA polymerase II transcription factor B subunit 2 (TFB2), partial CSB-YP762247DOT
CSB-EP762247DOT
CSB-BP762247DOT
CSB-MP762247DOT
CSB-EP762247DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Crossover junction endonuclease EME1 (EME1), partial CSB-YP762248DOT
CSB-EP762248DOT
CSB-BP762248DOT
CSB-MP762248DOT
CSB-EP762248DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 1 (MED1) CSB-YP762249DOT
CSB-EP762249DOT
CSB-BP762249DOT
CSB-MP762249DOT
CSB-EP762249DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Potential protein lysine methyltransferase SET5 (SET5) CSB-YP762250DOT
CSB-EP762250DOT
CSB-BP762250DOT
CSB-MP762250DOT
CSB-EP762250DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytoplasmic tRNA 2-thiolation protein 2 (NCS2) CSB-YP762251DOT
CSB-EP762251DOT
CSB-BP762251DOT
CSB-MP762251DOT
CSB-EP762251DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine hydroxymethyltransferase, cytosolic (SHM2) CSB-YP762252DOT
CSB-EP762252DOT
CSB-BP762252DOT
CSB-MP762252DOT
CSB-EP762252DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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