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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Guanine nucleotide-binding protein subunit gamma (ACR176C) CSB-YP762253DOT
CSB-EP762253DOT
CSB-BP762253DOT
CSB-MP762253DOT
CSB-EP762253DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Diacylglycerol O-acyltransferase 1 (DGA1), partial CSB-YP762254DOT1
CSB-EP762254DOT1
CSB-BP762254DOT1
CSB-MP762254DOT1
CSB-EP762254DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone H1 (HHOA) CSB-YP762255DOT
CSB-EP762255DOT
CSB-BP762255DOT
CSB-MP762255DOT
CSB-EP762255DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii NADH-cytochrome b5 reductase 2 (MCR1), partial CSB-YP762256DOT1
CSB-EP762256DOT1
CSB-BP762256DOT1
CSB-MP762256DOT1
CSB-EP762256DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone acetyltransferase type B subunit 2 (HAT2) CSB-YP762257DOT
CSB-EP762257DOT
CSB-BP762257DOT
CSB-MP762257DOT
CSB-EP762257DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone H2A.Z (HTZ1) CSB-YP762258DOT
CSB-EP762258DOT
CSB-BP762258DOT
CSB-MP762258DOT
CSB-EP762258DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine/threonine-protein kinase ATG1 (ATG1), partial CSB-YP762259DOT
CSB-EP762259DOT
CSB-BP762259DOT
CSB-MP762259DOT
CSB-EP762259DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Thioredoxin reductase (TRR1) CSB-YP762260DOT
CSB-EP762260DOT
CSB-BP762260DOT
CSB-MP762260DOT
CSB-EP762260DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytochrome b-c1 complex subunit 2, mitochondrial (QCR2) CSB-YP762261DOT
CSB-EP762261DOT
CSB-BP762261DOT
CSB-MP762261DOT
CSB-EP762261DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Guanine-nucleotide exchange factor YEL1 (YEL1), partial CSB-YP762262DOT
CSB-EP762262DOT
CSB-BP762262DOT
CSB-MP762262DOT
CSB-EP762262DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Altered inheritance of mitochondria protein 43, mitochondrial (AIM43), partial CSB-YP762263DOT1
CSB-EP762263DOT1
CSB-BP762263DOT1
CSB-MP762263DOT1
CSB-EP762263DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii rRNA-processing protein EFG1 (EFG1) CSB-YP762264DOT
CSB-EP762264DOT
CSB-BP762264DOT
CSB-MP762264DOT
CSB-EP762264DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Alcohol-sensitive RING finger protein 1 (ASR1) CSB-YP762265DOT
CSB-EP762265DOT
CSB-BP762265DOT
CSB-MP762265DOT
CSB-EP762265DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RNA polymerase II degradation factor 1 (DEF1), partial CSB-YP762266DOT
CSB-EP762266DOT
CSB-BP762266DOT
CSB-MP762266DOT
CSB-EP762266DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii mRNA turnover protein 4 homolog (MRT4) CSB-YP762267DOT
CSB-EP762267DOT
CSB-BP762267DOT
CSB-MP762267DOT
CSB-EP762267DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Flap endonuclease 1 (FEN1) CSB-YP762268DOT
CSB-EP762268DOT
CSB-BP762268DOT
CSB-MP762268DOT
CSB-EP762268DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 5-aminolevulinate synthase, mitochondrial (HEM1), partial CSB-YP762269DOT
CSB-EP762269DOT
CSB-BP762269DOT
CSB-MP762269DOT
CSB-EP762269DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone chaperone RTT106 (RTT106) CSB-YP762270DOT
CSB-EP762270DOT
CSB-BP762270DOT
CSB-MP762270DOT
CSB-EP762270DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 16 (SIN4), partial CSB-YP762271DOT
CSB-EP762271DOT
CSB-BP762271DOT
CSB-MP762271DOT
CSB-EP762271DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ubiquitin-like protein ATG12 (ATG12) CSB-YP762272DOT
CSB-EP762272DOT
CSB-BP762272DOT
CSB-MP762272DOT
CSB-EP762272DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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