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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa PKHD-type hydroxylase PD_1553 (PD_1553) CSB-YP801311XAT
CSB-EP801311XAT
CSB-BP801311XAT
CSB-MP801311XAT
CSB-EP801311XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Thiol:disulfide interchange protein DsbE (dsbE), partial CSB-YP801312XAT
CSB-EP801312XAT
CSB-BP801312XAT
CSB-MP801312XAT
CSB-EP801312XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Cytidylate kinase (cmk) CSB-YP801313XAT
CSB-EP801313XAT
CSB-BP801313XAT
CSB-MP801313XAT
CSB-EP801313XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Isoleucine--tRNA ligase (ileS), partial CSB-YP801314XAT
CSB-EP801314XAT
CSB-BP801314XAT
CSB-MP801314XAT
CSB-EP801314XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Beta-hexosaminidase (nagZ) CSB-YP801315XAT
CSB-EP801315XAT
CSB-BP801315XAT
CSB-MP801315XAT
CSB-EP801315XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Heat-inducible transcription repressor HrcA (hrcA) CSB-YP801316XAT
CSB-EP801316XAT
CSB-BP801316XAT
CSB-MP801316XAT
CSB-EP801316XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phosphoserine aminotransferase (serC) CSB-YP801317XAT
CSB-EP801317XAT
CSB-BP801317XAT
CSB-MP801317XAT
CSB-EP801317XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable chromosome-partitioning protein parB (parB) CSB-YP801318XAT
CSB-EP801318XAT
CSB-BP801318XAT
CSB-MP801318XAT
CSB-EP801318XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP801319XAT
CSB-EP801319XAT
CSB-BP801319XAT
CSB-MP801319XAT
CSB-EP801319XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP phosphoribosyltransferase (hisG) CSB-YP801320XAT
CSB-EP801320XAT
CSB-BP801320XAT
CSB-MP801320XAT
CSB-EP801320XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP801321XAT
CSB-EP801321XAT
CSB-BP801321XAT
CSB-MP801321XAT
CSB-EP801321XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Leucine--tRNA ligase (leuS), partial CSB-YP801322XAT
CSB-EP801322XAT
CSB-BP801322XAT
CSB-MP801322XAT
CSB-EP801322XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phosphate import ATP-binding protein PstB (pstB) CSB-YP801323XAT
CSB-EP801323XAT
CSB-BP801323XAT
CSB-MP801323XAT
CSB-EP801323XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein SlyX homolog (slyX) CSB-YP801324XAT
CSB-EP801324XAT
CSB-BP801324XAT
CSB-MP801324XAT
CSB-EP801324XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Type III pantothenate kinase (coaX) CSB-YP801325XAT
CSB-EP801325XAT
CSB-BP801325XAT
CSB-MP801325XAT
CSB-EP801325XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP801326XAT1
CSB-EP801326XAT1
CSB-BP801326XAT1
CSB-MP801326XAT1
CSB-EP801326XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Aspartate--tRNA ligase (aspS), partial CSB-YP801327XAT
CSB-EP801327XAT
CSB-BP801327XAT
CSB-MP801327XAT
CSB-EP801327XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable potassium transport system protein kup (kup), partial CSB-YP801328XAT
CSB-EP801328XAT
CSB-BP801328XAT
CSB-MP801328XAT
CSB-EP801328XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Quinolinate synthase A (nadA) CSB-YP801329XAT
CSB-EP801329XAT
CSB-BP801329XAT
CSB-MP801329XAT
CSB-EP801329XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP801330XAT
CSB-EP801330XAT
CSB-BP801330XAT
CSB-MP801330XAT
CSB-EP801330XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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