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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa Ribose-5-phosphate isomerase A (rpiA) CSB-YP801331XAT
CSB-EP801331XAT
CSB-BP801331XAT
CSB-MP801331XAT
CSB-EP801331XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S9 (rpsI) CSB-YP801332XAT
CSB-EP801332XAT
CSB-BP801332XAT
CSB-MP801332XAT
CSB-EP801332XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribonuclease PH (rph) CSB-YP801333XAT
CSB-EP801333XAT
CSB-BP801333XAT
CSB-MP801333XAT
CSB-EP801333XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP801334XAT
CSB-EP801334XAT
CSB-BP801334XAT
CSB-MP801334XAT
CSB-EP801334XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Diaminopimelate epimerase (dapF) CSB-YP801335XAT
CSB-EP801335XAT
CSB-BP801335XAT
CSB-MP801335XAT
CSB-EP801335XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP801336XAT
CSB-EP801336XAT
CSB-BP801336XAT
CSB-MP801336XAT
CSB-EP801336XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa HPr kinase/phosphorylase (hprK) CSB-YP801337XAT
CSB-EP801337XAT
CSB-BP801337XAT
CSB-MP801337XAT
CSB-EP801337XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Tryptophan synthase beta chain (trpB) CSB-YP801338XAT
CSB-EP801338XAT
CSB-BP801338XAT
CSB-MP801338XAT
CSB-EP801338XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protoheme IX farnesyltransferase (cyoE), partial CSB-YP801339XAT
CSB-EP801339XAT
CSB-BP801339XAT
CSB-MP801339XAT
CSB-EP801339XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Copper homeostasis protein CutC (cutC) CSB-YP801340XAT
CSB-EP801340XAT
CSB-BP801340XAT
CSB-MP801340XAT
CSB-EP801340XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP801341XAT
CSB-EP801341XAT
CSB-BP801341XAT
CSB-MP801341XAT
CSB-EP801341XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Trigger factor (tig) CSB-YP801342XAT
CSB-EP801342XAT
CSB-BP801342XAT
CSB-MP801342XAT
CSB-EP801342XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L15 (rplO) CSB-YP801343XAT
CSB-EP801343XAT
CSB-BP801343XAT
CSB-MP801343XAT
CSB-EP801343XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L5 (rplE) CSB-YP801344XAT
CSB-EP801344XAT
CSB-BP801344XAT
CSB-MP801344XAT
CSB-EP801344XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L2 (rplB) CSB-YP801345XAT
CSB-EP801345XAT
CSB-BP801345XAT
CSB-MP801345XAT
CSB-EP801345XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP synthase subunit alpha (atpA), partial CSB-YP801346XAT
CSB-EP801346XAT
CSB-BP801346XAT
CSB-MP801346XAT
CSB-EP801346XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Maf-like protein PD_0415 (PD_0415) CSB-YP801347XAT
CSB-EP801347XAT
CSB-BP801347XAT
CSB-MP801347XAT
CSB-EP801347XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP801348XAT1
CSB-EP801348XAT1
CSB-BP801348XAT1
CSB-MP801348XAT1
CSB-EP801348XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP801349XAT
CSB-EP801349XAT
CSB-BP801349XAT
CSB-MP801349XAT
CSB-EP801349XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable manganese transport protein MntH (mntH), partial CSB-YP801350XAT
CSB-EP801350XAT
CSB-BP801350XAT
CSB-MP801350XAT
CSB-EP801350XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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