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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis biovar 1 Proline--tRNA ligase (proS) CSB-YP812671BMQ
CSB-EP812671BMQ
CSB-BP812671BMQ
CSB-MP812671BMQ
CSB-EP812671BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP812672BMQ
CSB-EP812672BMQ
CSB-BP812672BMQ
CSB-MP812672BMQ
CSB-EP812672BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 6,7-dimethyl-8-ribityllumazine synthase 1 (ribH1) CSB-YP812673BMQ
CSB-EP812673BMQ
CSB-BP812673BMQ
CSB-MP812673BMQ
CSB-EP812673BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP812674BMQ
CSB-EP812674BMQ
CSB-BP812674BMQ
CSB-MP812674BMQ
CSB-EP812674BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP812675BMQ
CSB-EP812675BMQ
CSB-BP812675BMQ
CSB-MP812675BMQ
CSB-EP812675BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 5-hydroxyisourate hydrolase (BR0508, BS1330_I0509) CSB-YP812676BMQ
CSB-EP812676BMQ
CSB-BP812676BMQ
CSB-MP812676BMQ
CSB-EP812676BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Na (+)/H (+) antiporter nhaA (nhaA), partial CSB-YP812677BMQ
CSB-EP812677BMQ
CSB-BP812677BMQ
CSB-MP812677BMQ
CSB-EP812677BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Probable multidrug resistance protein norM (norM), partial CSB-YP812678BMQ
CSB-EP812678BMQ
CSB-BP812678BMQ
CSB-MP812678BMQ
CSB-EP812678BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Protein ApaG (apaG) CSB-YP812679BMQ
CSB-EP812679BMQ
CSB-BP812679BMQ
CSB-MP812679BMQ
CSB-EP812679BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Urease accessory protein UreF 1 (ureF1) CSB-YP812680BMQ
CSB-EP812680BMQ
CSB-BP812680BMQ
CSB-MP812680BMQ
CSB-EP812680BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Urease accessory protein UreD 1 (ureD1) CSB-YP812681BMQ
CSB-EP812681BMQ
CSB-BP812681BMQ
CSB-MP812681BMQ
CSB-EP812681BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Integration host factor subunit beta (ihfB) CSB-YP812682BMQ
CSB-EP812682BMQ
CSB-BP812682BMQ
CSB-MP812682BMQ
CSB-EP812682BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Error-prone DNA polymerase (dnaE2), partial CSB-YP812683BMQ
CSB-EP812683BMQ
CSB-BP812683BMQ
CSB-MP812683BMQ
CSB-EP812683BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Chromosomal replication initiator protein DnaA (dnaA) CSB-YP812684BMQ
CSB-EP812684BMQ
CSB-BP812684BMQ
CSB-MP812684BMQ
CSB-EP812684BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP812685BGP
CSB-EP812685BGP
CSB-BP812685BGP
CSB-MP812685BGP
CSB-EP812685BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Ribosome-binding factor A (rbfA) CSB-YP812686BGP
CSB-EP812686BGP
CSB-BP812686BGP
CSB-MP812686BGP
CSB-EP812686BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L6 (rplF) CSB-YP812687BGP
CSB-EP812687BGP
CSB-BP812687BGP
CSB-MP812687BGP
CSB-EP812687BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L16 (rplP) CSB-YP812688BGP
CSB-EP812688BGP
CSB-BP812688BGP
CSB-MP812688BGP
CSB-EP812688BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 30S ribosomal protein S10 (rpsJ) CSB-YP812689BGP
CSB-EP812689BGP
CSB-BP812689BGP
CSB-MP812689BGP
CSB-EP812689BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Leucine--tRNA ligase (leuS), partial CSB-YP812690BGP
CSB-EP812690BGP
CSB-BP812690BGP
CSB-MP812690BGP
CSB-EP812690BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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