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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bifidobacterium longum Uncharacterized RNA methyltransferase BL1394 (BL1394) CSB-YP812691BGP
CSB-EP812691BGP
CSB-BP812691BGP
CSB-MP812691BGP
CSB-EP812691BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Transcriptional repressor NrdR (nrdR) CSB-YP812692BGP
CSB-EP812692BGP
CSB-BP812692BGP
CSB-MP812692BGP
CSB-EP812692BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Histidinol dehydrogenase (hisD) CSB-YP812693BGP
CSB-EP812693BGP
CSB-BP812693BGP
CSB-MP812693BGP
CSB-EP812693BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Arginine--tRNA ligase (argS), partial CSB-YP812694BGP
CSB-EP812694BGP
CSB-BP812694BGP
CSB-MP812694BGP
CSB-EP812694BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 3-isopropylmalate dehydrogenase (leuB) CSB-YP812695BGP
CSB-EP812695BGP
CSB-BP812695BGP
CSB-MP812695BGP
CSB-EP812695BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Deoxyguanosinetriphosphate triphosphohydrolase-like protein (dgt) CSB-YP812696BGP
CSB-EP812696BGP
CSB-BP812696BGP
CSB-MP812696BGP
CSB-EP812696BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP812697BGP
CSB-EP812697BGP
CSB-BP812697BGP
CSB-MP812697BGP
CSB-EP812697BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Arginine repressor (argR) CSB-YP812698BGP
CSB-EP812698BGP
CSB-BP812698BGP
CSB-MP812698BGP
CSB-EP812698BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Gamma-glutamyl phosphate reductase (proA) CSB-YP812699BGP
CSB-EP812699BGP
CSB-BP812699BGP
CSB-MP812699BGP
CSB-EP812699BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum UvrABC system protein B (uvrB), partial CSB-YP812700BGP
CSB-EP812700BGP
CSB-BP812700BGP
CSB-MP812700BGP
CSB-EP812700BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP812701BGP
CSB-EP812701BGP
CSB-BP812701BGP
CSB-MP812701BGP
CSB-EP812701BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Elongation factor 4 (lepA), partial CSB-YP812702BGP
CSB-EP812702BGP
CSB-BP812702BGP
CSB-MP812702BGP
CSB-EP812702BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP812703BGP
CSB-EP812703BGP
CSB-BP812703BGP
CSB-MP812703BGP
CSB-EP812703BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Triosephosphate isomerase (tpiA) CSB-YP812704BGP
CSB-EP812704BGP
CSB-BP812704BGP
CSB-MP812704BGP
CSB-EP812704BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Membrane protein insertase YidC (yidC), partial CSB-YP812705BGP1
CSB-EP812705BGP1
CSB-BP812705BGP1
CSB-MP812705BGP1
CSB-EP812705BGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum UPF0233 membrane protein BL0593 (BL0593), partial CSB-YP812706BGP1
CSB-EP812706BGP1
CSB-BP812706BGP1
CSB-MP812706BGP1
CSB-EP812706BGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Chaperone protein DnaK (dnaK), partial CSB-YP812707BGP
CSB-EP812707BGP
CSB-BP812707BGP
CSB-MP812707BGP
CSB-EP812707BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 30S ribosomal protein S18 (rpsR) CSB-YP812708BGP
CSB-EP812708BGP
CSB-BP812708BGP
CSB-MP812708BGP
CSB-EP812708BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum ATP synthase subunit c (atpE), partial CSB-YP812709BGP1
CSB-EP812709BGP1
CSB-BP812709BGP1
CSB-MP812709BGP1
CSB-EP812709BGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum ATP synthase gamma chain (atpG) CSB-YP812710BGP
CSB-EP812710BGP
CSB-BP812710BGP
CSB-MP812710BGP
CSB-EP812710BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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