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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis biovar 1 ATP synthase subunit b 2 (atpF2), partial CSB-YP815356BMQ
CSB-EP815356BMQ
CSB-BP815356BMQ
CSB-MP815356BMQ
CSB-EP815356BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Efflux pump periplasmic linker BepD (bepD) CSB-YP815357BMQ
CSB-EP815357BMQ
CSB-BP815357BMQ
CSB-MP815357BMQ
CSB-EP815357BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Thiazole synthase (thiG) CSB-YP815358BMQ
CSB-EP815358BMQ
CSB-BP815358BMQ
CSB-MP815358BMQ
CSB-EP815358BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Argininosuccinate synthase (argG) CSB-YP815359BMQ
CSB-EP815359BMQ
CSB-BP815359BMQ
CSB-MP815359BMQ
CSB-EP815359BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP815360BMQ
CSB-EP815360BMQ
CSB-BP815360BMQ
CSB-MP815360BMQ
CSB-EP815360BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum S-adenosylmethionine synthase (metK) CSB-YP815361BGP
CSB-EP815361BGP
CSB-BP815361BGP
CSB-MP815361BGP
CSB-EP815361BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L31 (rpmE) CSB-YP815362BGP
CSB-EP815362BGP
CSB-BP815362BGP
CSB-MP815362BGP
CSB-EP815362BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Thymidylate synthase (thyA) CSB-YP815363BGP
CSB-EP815363BGP
CSB-BP815363BGP
CSB-MP815363BGP
CSB-EP815363BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Translation initiation factor IF-2 (infB), partial CSB-YP815364BGP
CSB-EP815364BGP
CSB-BP815364BGP
CSB-MP815364BGP
CSB-EP815364BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L18 (rplR) CSB-YP815365BGP
CSB-EP815365BGP
CSB-BP815365BGP
CSB-MP815365BGP
CSB-EP815365BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L14 (rplN) CSB-YP815366BGP
CSB-EP815366BGP
CSB-BP815366BGP
CSB-MP815366BGP
CSB-EP815366BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L33 (rpmG) CSB-YP815367BGP
CSB-EP815367BGP
CSB-BP815367BGP
CSB-MP815367BGP
CSB-EP815367BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 30S ribosomal protein S15 (rpsO) CSB-YP815368BGP
CSB-EP815368BGP
CSB-BP815368BGP
CSB-MP815368BGP
CSB-EP815368BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP815369BGP
CSB-EP815369BGP
CSB-BP815369BGP
CSB-MP815369BGP
CSB-EP815369BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L20 (rplT) CSB-YP815370BGP
CSB-EP815370BGP
CSB-BP815370BGP
CSB-MP815370BGP
CSB-EP815370BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Glucosamine-6-phosphate deaminase (nagB) CSB-YP815371BGP
CSB-EP815371BGP
CSB-BP815371BGP
CSB-MP815371BGP
CSB-EP815371BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum LexA repressor (lexA) CSB-YP815372BGP
CSB-EP815372BGP
CSB-BP815372BGP
CSB-MP815372BGP
CSB-EP815372BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum GTPase obg (obg), partial CSB-YP815373BGP
CSB-EP815373BGP
CSB-BP815373BGP
CSB-MP815373BGP
CSB-EP815373BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP815374BGP
CSB-EP815374BGP
CSB-BP815374BGP
CSB-MP815374BGP
CSB-EP815374BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Dihydrodipicolinate synthase (dapA) CSB-YP815375BGP
CSB-EP815375BGP
CSB-BP815375BGP
CSB-MP815375BGP
CSB-EP815375BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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