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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus epidermidis Probable thiol peroxidase (tpx) CSB-YP815958SLD
CSB-EP815958SLD
CSB-BP815958SLD
CSB-MP815958SLD
CSB-EP815958SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Transcriptional repressor NrdR (nrdR) CSB-YP815959SLD
CSB-EP815959SLD
CSB-BP815959SLD
CSB-MP815959SLD
CSB-EP815959SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP815960SLD
CSB-EP815960SLD
CSB-BP815960SLD
CSB-MP815960SLD
CSB-EP815960SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Transcription elongation factor GreA (greA) CSB-YP815961SLD
CSB-EP815961SLD
CSB-BP815961SLD
CSB-MP815961SLD
CSB-EP815961SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Ribosomal protein L11 methyltransferase (prmA) CSB-YP815962SLD
CSB-EP815962SLD
CSB-BP815962SLD
CSB-MP815962SLD
CSB-EP815962SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Shikimate kinase (aroK) CSB-YP815963SLD
CSB-EP815963SLD
CSB-BP815963SLD
CSB-MP815963SLD
CSB-EP815963SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Asparagine--tRNA ligase (asnS) CSB-YP815964SLD
CSB-EP815964SLD
CSB-BP815964SLD
CSB-MP815964SLD
CSB-EP815964SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Anthranilate phosphoribosyltransferase (trpD) CSB-YP815965SLD
CSB-EP815965SLD
CSB-BP815965SLD
CSB-MP815965SLD
CSB-EP815965SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0291 protein SE_1024 (SE_1024) CSB-YP815966SLD
CSB-EP815966SLD
CSB-BP815966SLD
CSB-MP815966SLD
CSB-EP815966SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glycerol kinase (glpK) CSB-YP815967SLD
CSB-EP815967SLD
CSB-BP815967SLD
CSB-MP815967SLD
CSB-EP815967SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Ribosome maturation factor RimP (rimP) CSB-YP815968SLD
CSB-EP815968SLD
CSB-BP815968SLD
CSB-MP815968SLD
CSB-EP815968SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0122 protein SE_0911 (SE_0911) CSB-YP815969SLD
CSB-EP815969SLD
CSB-BP815969SLD
CSB-MP815969SLD
CSB-EP815969SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP815970SLD
CSB-EP815970SLD
CSB-BP815970SLD
CSB-MP815970SLD
CSB-EP815970SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP815971SLD
CSB-EP815971SLD
CSB-BP815971SLD
CSB-MP815971SLD
CSB-EP815971SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (pdhC) CSB-YP815972SLD
CSB-EP815972SLD
CSB-BP815972SLD
CSB-MP815972SLD
CSB-EP815972SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0477 protein SE_0715 (SE_0715) CSB-YP815973SLD
CSB-EP815973SLD
CSB-BP815973SLD
CSB-MP815973SLD
CSB-EP815973SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0344 protein SE_0666 (SE_0666), partial CSB-YP815974SLD1
CSB-EP815974SLD1
CSB-BP815974SLD1
CSB-MP815974SLD1
CSB-EP815974SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP815975SLD
CSB-EP815975SLD
CSB-BP815975SLD
CSB-MP815975SLD
CSB-EP815975SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP815976SLD1
CSB-EP815976SLD1
CSB-BP815976SLD1
CSB-MP815976SLD1
CSB-EP815976SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Sensor histidine kinase graS (graS), partial CSB-YP815977SLD1
CSB-EP815977SLD1
CSB-BP815977SLD1
CSB-MP815977SLD1
CSB-EP815977SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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