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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bifidobacterium longum Trigger factor (tig) CSB-YP818063BGP
CSB-EP818063BGP
CSB-BP818063BGP
CSB-MP818063BGP
CSB-EP818063BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP818064BGP
CSB-EP818064BGP
CSB-BP818064BGP
CSB-MP818064BGP
CSB-EP818064BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP818065BGP
CSB-EP818065BGP
CSB-BP818065BGP
CSB-MP818065BGP
CSB-EP818065BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L25 (rplY) CSB-YP818066BGP
CSB-EP818066BGP
CSB-BP818066BGP
CSB-MP818066BGP
CSB-EP818066BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Aspartate carbamoyltransferase (pyrB) CSB-YP818067BGP
CSB-EP818067BGP
CSB-BP818067BGP
CSB-MP818067BGP
CSB-EP818067BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP818068BGP
CSB-EP818068BGP
CSB-BP818068BGP
CSB-MP818068BGP
CSB-EP818068BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP818069BGP
CSB-EP818069BGP
CSB-BP818069BGP
CSB-MP818069BGP
CSB-EP818069BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Phosphoglycerate kinase (pgk) CSB-YP818070BGP
CSB-EP818070BGP
CSB-BP818070BGP
CSB-MP818070BGP
CSB-EP818070BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP818071BGP
CSB-EP818071BGP
CSB-BP818071BGP
CSB-MP818071BGP
CSB-EP818071BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Putative membrane protein insertion efficiency factor (BL0643) CSB-YP818072BGP
CSB-EP818072BGP
CSB-BP818072BGP
CSB-MP818072BGP
CSB-EP818072BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Ketol-acid reductoisomerase 2 (ilvC2) CSB-YP818073BGP
CSB-EP818073BGP
CSB-BP818073BGP
CSB-MP818073BGP
CSB-EP818073BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Dipeptidase (pepD), partial CSB-YP818074BGP
CSB-EP818074BGP
CSB-BP818074BGP
CSB-MP818074BGP
CSB-EP818074BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 30S ribosomal protein S6 (rpsF) CSB-YP818075BGP
CSB-EP818075BGP
CSB-BP818075BGP
CSB-MP818075BGP
CSB-EP818075BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP818076BGP
CSB-EP818076BGP
CSB-BP818076BGP
CSB-MP818076BGP
CSB-EP818076BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum D-alanine--D-alanine ligase (ddl) CSB-YP818077BGP
CSB-EP818077BGP
CSB-BP818077BGP
CSB-MP818077BGP
CSB-EP818077BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Cysteine--tRNA ligase (cysS), partial CSB-YP818078BGP
CSB-EP818078BGP
CSB-BP818078BGP
CSB-MP818078BGP
CSB-EP818078BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Guanylate kinase (gmk) CSB-YP818079BGP
CSB-EP818079BGP
CSB-BP818079BGP
CSB-MP818079BGP
CSB-EP818079BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Carbamoyl-phosphate synthase small chain (carA) CSB-YP818080BGP
CSB-EP818080BGP
CSB-BP818080BGP
CSB-MP818080BGP
CSB-EP818080BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Aspartate--tRNA ligase (aspS), partial CSB-YP818081BGP
CSB-EP818081BGP
CSB-BP818081BGP
CSB-MP818081BGP
CSB-EP818081BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydroxylamine reductase (hcp), partial CSB-YP818082PIF
CSB-EP818082PIF
CSB-BP818082PIF
CSB-MP818082PIF
CSB-EP818082PIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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