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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Small RNA degrading nuclease 5 (SDN5), partial CSB-YP818504DOA
CSB-EP818504DOA
CSB-BP818504DOA
CSB-MP818504DOA
CSB-EP818504DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Microtubule-associated protein 70-2 (MAP70.2), partial CSB-YP818505DOA
CSB-EP818505DOA
CSB-BP818505DOA
CSB-MP818505DOA
CSB-EP818505DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein phosphatase BSL1 (BSL1), partial CSB-YP818506DOA
CSB-EP818506DOA
CSB-BP818506DOA
CSB-MP818506DOA
CSB-EP818506DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g22160 (At4g22160) CSB-YP818507DOA
CSB-EP818507DOA
CSB-BP818507DOA
CSB-MP818507DOA
CSB-EP818507DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Inosine triphosphate pyrophosphatase (At4g13720) CSB-YP818508DOA
CSB-EP818508DOA
CSB-BP818508DOA
CSB-MP818508DOA
CSB-EP818508DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L18a-1 (RPL18AA) CSB-YP818509DOA
CSB-EP818509DOA
CSB-BP818509DOA
CSB-MP818509DOA
CSB-EP818509DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histidine-containing phosphotransfer protein 5 (AHP5) CSB-YP818510DOA
CSB-EP818510DOA
CSB-BP818510DOA
CSB-MP818510DOA
CSB-EP818510DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative phytosulfokines 6 (PSK6) CSB-YP818511DOA
CSB-EP818511DOA
CSB-BP818511DOA
CSB-MP818511DOA
CSB-EP818511DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein SCO1 homolog 2, mitochondrial (HCC2), partial CSB-YP818512DOA1
CSB-EP818512DOA1
CSB-BP818512DOA1
CSB-MP818512DOA1
CSB-EP818512DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcineurin B-like protein 2 (CBL2) CSB-YP818513DOA
CSB-EP818513DOA
CSB-BP818513DOA
CSB-MP818513DOA
CSB-EP818513DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein REM20 (REM20) CSB-YP818514DOA
CSB-EP818514DOA
CSB-BP818514DOA
CSB-MP818514DOA
CSB-EP818514DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Homocysteine S-methyltransferase 3 (HMT3) CSB-YP818515DOA
CSB-EP818515DOA
CSB-BP818515DOA
CSB-MP818515DOA
CSB-EP818515DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 69 (At5g10740) CSB-YP818516DOA
CSB-EP818516DOA
CSB-BP818516DOA
CSB-MP818516DOA
CSB-EP818516DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NEP1-interacting protein-like 2 (ATL24), partial CSB-YP818517DOA1
CSB-EP818517DOA1
CSB-BP818517DOA1
CSB-MP818517DOA1
CSB-EP818517DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1 (PIS1), partial CSB-YP818518DOA1
CSB-EP818518DOA1
CSB-BP818518DOA1
CSB-MP818518DOA1
CSB-EP818518DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative clathrin assembly protein At2g01600 (At2g01600), partial CSB-YP818519DOA
CSB-EP818519DOA
CSB-BP818519DOA
CSB-MP818519DOA
CSB-EP818519DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana SPX domain-containing protein 1 (SPX1) CSB-YP818520DOA
CSB-EP818520DOA
CSB-BP818520DOA
CSB-MP818520DOA
CSB-EP818520DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L5-1 (ATL5) CSB-YP818521DOA
CSB-EP818521DOA
CSB-BP818521DOA
CSB-MP818521DOA
CSB-EP818521DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Monothiol glutaredoxin-S15, mitochondrial (GRXS15) CSB-YP818522DOA
CSB-EP818522DOA
CSB-BP818522DOA
CSB-MP818522DOA
CSB-EP818522DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein TIFY 5A (TIFY5A) CSB-YP818523DOA
CSB-EP818523DOA
CSB-BP818523DOA
CSB-MP818523DOA
CSB-EP818523DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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