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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Rhizobium meliloti Urease accessory protein UreE (ureE) CSB-YP821840RKU
CSB-EP821840RKU
CSB-BP821840RKU
CSB-MP821840RKU
CSB-EP821840RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP821841RKU
CSB-EP821841RKU
CSB-BP821841RKU
CSB-MP821841RKU
CSB-EP821841RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Triosephosphate isomerase 2 (tpiA2) CSB-YP821842RKU
CSB-EP821842RKU
CSB-BP821842RKU
CSB-MP821842RKU
CSB-EP821842RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Aquaporin Z 1 (aqpZ1), partial CSB-YP821843RKU1
CSB-EP821843RKU1
CSB-BP821843RKU1
CSB-MP821843RKU1
CSB-EP821843RKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP821844RKU
CSB-EP821844RKU
CSB-BP821844RKU
CSB-MP821844RKU
CSB-EP821844RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP821845RKU
CSB-EP821845RKU
CSB-BP821845RKU
CSB-MP821845RKU
CSB-EP821845RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 30S ribosomal protein S4 (rpsD) CSB-YP821846RKU
CSB-EP821846RKU
CSB-BP821846RKU
CSB-MP821846RKU
CSB-EP821846RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti UPF0758 protein R01728 (R01728) CSB-YP821847RKU
CSB-EP821847RKU
CSB-BP821847RKU
CSB-MP821847RKU
CSB-EP821847RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti LexA repressor (lexA) CSB-YP821848RKU
CSB-EP821848RKU
CSB-BP821848RKU
CSB-MP821848RKU
CSB-EP821848RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Glycine cleavage system H protein (gcvH) CSB-YP821849RKU
CSB-EP821849RKU
CSB-BP821849RKU
CSB-MP821849RKU
CSB-EP821849RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Deoxyguanosinetriphosphate triphosphohydrolase-like protein (R01522) CSB-YP821850RKU
CSB-EP821850RKU
CSB-BP821850RKU
CSB-MP821850RKU
CSB-EP821850RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Ribosome-recycling factor (frr) CSB-YP821851RKU
CSB-EP821851RKU
CSB-BP821851RKU
CSB-MP821851RKU
CSB-EP821851RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Enolase (eno) CSB-YP821852RKU
CSB-EP821852RKU
CSB-BP821852RKU
CSB-MP821852RKU
CSB-EP821852RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti UPF0229 protein R01398 (R01398) CSB-YP821853RKU
CSB-EP821853RKU
CSB-BP821853RKU
CSB-MP821853RKU
CSB-EP821853RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 50S ribosomal protein L30 (rpmD) CSB-YP821854RKU
CSB-EP821854RKU
CSB-BP821854RKU
CSB-MP821854RKU
CSB-EP821854RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 50S ribosomal protein L24 (rplX) CSB-YP821855RKU
CSB-EP821855RKU
CSB-BP821855RKU
CSB-MP821855RKU
CSB-EP821855RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 50S ribosomal protein L23 (rplW) CSB-YP821856RKU
CSB-EP821856RKU
CSB-BP821856RKU
CSB-MP821856RKU
CSB-EP821856RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP821857RKU
CSB-EP821857RKU
CSB-BP821857RKU
CSB-MP821857RKU
CSB-EP821857RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP821858RKU
CSB-EP821858RKU
CSB-BP821858RKU
CSB-MP821858RKU
CSB-EP821858RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 50S ribosomal protein L33 (rpmG) CSB-YP821859RKU
CSB-EP821859RKU
CSB-BP821859RKU
CSB-MP821859RKU
CSB-EP821859RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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