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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serovar 1/2a Listeria nuclear targeted protein A (lntA) CSB-YP835048LPY
CSB-EP835048LPY
CSB-BP835048LPY
CSB-MP835048LPY
CSB-EP835048LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable transcriptional regulatory protein lmo0369 (lmo0369) CSB-YP835049LPY
CSB-EP835049LPY
CSB-BP835049LPY
CSB-MP835049LPY
CSB-EP835049LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a 50S ribosomal protein L7/L12 (rplL) CSB-YP835050LPY
CSB-EP835050LPY
CSB-BP835050LPY
CSB-MP835050LPY
CSB-EP835050LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Lysine--tRNA ligase (lysS) CSB-YP835051LPY
CSB-EP835051LPY
CSB-BP835051LPY
CSB-MP835051LPY
CSB-EP835051LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Methionine--tRNA ligase (metG), partial CSB-YP835052LPY
CSB-EP835052LPY
CSB-BP835052LPY
CSB-MP835052LPY
CSB-EP835052LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a ATP synthase subunit beta 1 (atpD1) CSB-YP835053LPY
CSB-EP835053LPY
CSB-BP835053LPY
CSB-MP835053LPY
CSB-EP835053LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Arginine deiminase (arcA) CSB-YP835054LPY
CSB-EP835054LPY
CSB-BP835054LPY
CSB-MP835054LPY
CSB-EP835054LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Flagellar L-ring protein (flgH) CSB-YP835055BMO
CSB-EP835055BMO
CSB-BP835055BMO
CSB-MP835055BMO
CSB-EP835055BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Probable septum site-determining protein MinC (minC) CSB-YP835056BMO
CSB-EP835056BMO
CSB-BP835056BMO
CSB-MP835056BMO
CSB-EP835056BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Putative peptide import ATP-binding protein BMEII0864 (BMEII0864) CSB-YP835057BMO
CSB-EP835057BMO
CSB-BP835057BMO
CSB-MP835057BMO
CSB-EP835057BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Homoserine O-succinyltransferase (metA) CSB-YP835058BMO
CSB-EP835058BMO
CSB-BP835058BMO
CSB-MP835058BMO
CSB-EP835058BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Blue-light-activated histidine kinase (BMEII0679) CSB-YP835059BMO
CSB-EP835059BMO
CSB-BP835059BMO
CSB-MP835059BMO
CSB-EP835059BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 sn-glycerol-3-phosphate-binding periplasmic protein ugpB (ugpB) CSB-YP835060BMO
CSB-EP835060BMO
CSB-BP835060BMO
CSB-MP835060BMO
CSB-EP835060BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Protein FdhD homolog (fdhD) CSB-YP835061BMO
CSB-EP835061BMO
CSB-BP835061BMO
CSB-MP835061BMO
CSB-EP835061BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Nickel import ATP-binding protein NikD (nikD) CSB-YP835062BMO
CSB-EP835062BMO
CSB-BP835062BMO
CSB-MP835062BMO
CSB-EP835062BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP835063BMO
CSB-EP835063BMO
CSB-BP835063BMO
CSB-MP835063BMO
CSB-EP835063BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Bifunctional imidazolonepropionase/histidine ammonia-lyase (hutIH), partial CSB-YP835064BMO
CSB-EP835064BMO
CSB-BP835064BMO
CSB-MP835064BMO
CSB-EP835064BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Acetylglutamate kinase (argB) CSB-YP835065BMO
CSB-EP835065BMO
CSB-BP835065BMO
CSB-MP835065BMO
CSB-EP835065BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Uracil phosphoribosyltransferase (upp) CSB-YP835066BMO
CSB-EP835066BMO
CSB-BP835066BMO
CSB-MP835066BMO
CSB-EP835066BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Putative peptide import ATP-binding protein BMEII0205 (BMEII0205) CSB-YP835067BMO
CSB-EP835067BMO
CSB-BP835067BMO
CSB-MP835067BMO
CSB-EP835067BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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