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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Corynebacterium glutamicum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP836821DWY
CSB-EP836821DWY
CSB-BP836821DWY
CSB-MP836821DWY
CSB-EP836821DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP836822DWY
CSB-EP836822DWY
CSB-BP836822DWY
CSB-MP836822DWY
CSB-EP836822DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Probable thiol peroxidase (tpx) CSB-YP836823DWY
CSB-EP836823DWY
CSB-BP836823DWY
CSB-MP836823DWY
CSB-EP836823DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Pantothenate kinase (coaA) CSB-YP836824DWY
CSB-EP836824DWY
CSB-BP836824DWY
CSB-MP836824DWY
CSB-EP836824DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glucose-6-phosphate isomerase (pgi), partial CSB-YP836825DWY
CSB-EP836825DWY
CSB-BP836825DWY
CSB-MP836825DWY
CSB-EP836825DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum HTH-type pyridoxine biosynthesis transcriptional regulator pdxR (pdxR) CSB-YP836826DWY
CSB-EP836826DWY
CSB-BP836826DWY
CSB-MP836826DWY
CSB-EP836826DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 2-methylcitrate synthase 1 (prpC1) CSB-YP836827DWY
CSB-EP836827DWY
CSB-BP836827DWY
CSB-MP836827DWY
CSB-EP836827DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Probable methylisocitrate lyase 2 (prpB2) CSB-YP836828DWY
CSB-EP836828DWY
CSB-BP836828DWY
CSB-MP836828DWY
CSB-EP836828DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Methionine import ATP-binding protein MetN (metN) CSB-YP836829DWY
CSB-EP836829DWY
CSB-BP836829DWY
CSB-MP836829DWY
CSB-EP836829DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP836830DWY
CSB-EP836830DWY
CSB-BP836830DWY
CSB-MP836830DWY
CSB-EP836830DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L18 (rplR) CSB-YP836831DWY
CSB-EP836831DWY
CSB-BP836831DWY
CSB-MP836831DWY
CSB-EP836831DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L16 (rplP) CSB-YP836832DWY
CSB-EP836832DWY
CSB-BP836832DWY
CSB-MP836832DWY
CSB-EP836832DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L3 (rplC) CSB-YP836833DWY
CSB-EP836833DWY
CSB-BP836833DWY
CSB-MP836833DWY
CSB-EP836833DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP836834DWY
CSB-EP836834DWY
CSB-BP836834DWY
CSB-MP836834DWY
CSB-EP836834DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP836835DWY
CSB-EP836835DWY
CSB-BP836835DWY
CSB-MP836835DWY
CSB-EP836835DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Putative 3-methyladenine DNA glycosylase (mag) CSB-YP836836DWY
CSB-EP836836DWY
CSB-BP836836DWY
CSB-MP836836DWY
CSB-EP836836DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0176 protein MW2609 (MW2609) CSB-YP836837SUL
CSB-EP836837SUL
CSB-BP836837SUL
CSB-MP836837SUL
CSB-EP836837SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP836838SUL
CSB-EP836838SUL
CSB-BP836838SUL
CSB-MP836838SUL
CSB-EP836838SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Clumping factor B (clfB), partial CSB-YP836839SUL
CSB-EP836839SUL
CSB-BP836839SUL
CSB-MP836839SUL
CSB-EP836839SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dehydrosqualene synthase (crtM) CSB-YP836840SUL
CSB-EP836840SUL
CSB-BP836840SUL
CSB-MP836840SUL
CSB-EP836840SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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