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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizobium meliloti Glutamate 5-kinase 2 (proB2) CSB-YP838943RKU
CSB-EP838943RKU
CSB-BP838943RKU
CSB-MP838943RKU
CSB-EP838943RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti ATP synthase subunit alpha (atpA), partial CSB-YP838944RKU
CSB-EP838944RKU
CSB-BP838944RKU
CSB-MP838944RKU
CSB-EP838944RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Methionine import ATP-binding protein MetN (metN) CSB-YP838945RKU
CSB-EP838945RKU
CSB-BP838945RKU
CSB-MP838945RKU
CSB-EP838945RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Outer membrane lipoprotein omp10 homolog (omp10) CSB-YP838946RKU
CSB-EP838946RKU
CSB-BP838946RKU
CSB-MP838946RKU
CSB-EP838946RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP838947RKU
CSB-EP838947RKU
CSB-BP838947RKU
CSB-MP838947RKU
CSB-EP838947RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 3-dehydroquinate synthase (aroB) CSB-YP838948RKU
CSB-EP838948RKU
CSB-BP838948RKU
CSB-MP838948RKU
CSB-EP838948RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Chaperone protein ClpB (clpB), partial CSB-YP838949RKU
CSB-EP838949RKU
CSB-BP838949RKU
CSB-MP838949RKU
CSB-EP838949RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Urease accessory protein UreG (ureG) CSB-YP838950RKU
CSB-EP838950RKU
CSB-BP838950RKU
CSB-MP838950RKU
CSB-EP838950RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Peptidyl-tRNA hydrolase (pth) CSB-YP838951RKU
CSB-EP838951RKU
CSB-BP838951RKU
CSB-MP838951RKU
CSB-EP838951RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP838952RKU
CSB-EP838952RKU
CSB-BP838952RKU
CSB-MP838952RKU
CSB-EP838952RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Pantothenate synthetase (panC) CSB-YP838953RKU
CSB-EP838953RKU
CSB-BP838953RKU
CSB-MP838953RKU
CSB-EP838953RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Cobyrinic acid A,C-diamide synthase (cobB) CSB-YP838954RKU
CSB-EP838954RKU
CSB-BP838954RKU
CSB-MP838954RKU
CSB-EP838954RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Fumarate hydratase class II (fumC) CSB-YP838955RKU
CSB-EP838955RKU
CSB-BP838955RKU
CSB-MP838955RKU
CSB-EP838955RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP838956RKU
CSB-EP838956RKU
CSB-BP838956RKU
CSB-MP838956RKU
CSB-EP838956RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Anthranilate phosphoribosyltransferase (trpD) CSB-YP838957RKU
CSB-EP838957RKU
CSB-BP838957RKU
CSB-MP838957RKU
CSB-EP838957RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Queuine tRNA-ribosyltransferase (tgt) CSB-YP838958RKU
CSB-EP838958RKU
CSB-BP838958RKU
CSB-MP838958RKU
CSB-EP838958RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP838959RKU
CSB-EP838959RKU
CSB-BP838959RKU
CSB-MP838959RKU
CSB-EP838959RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP838960RKU
CSB-EP838960RKU
CSB-BP838960RKU
CSB-MP838960RKU
CSB-EP838960RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Protein hfq (hfq) CSB-YP838961RKU
CSB-EP838961RKU
CSB-BP838961RKU
CSB-MP838961RKU
CSB-EP838961RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Putative membrane protein insertion efficiency factor (R01422) CSB-YP838962RKU
CSB-EP838962RKU
CSB-BP838962RKU
CSB-MP838962RKU
CSB-EP838962RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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