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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP839969SUL
CSB-EP839969SUL
CSB-BP839969SUL
CSB-MP839969SUL
CSB-EP839969SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable malate:quinone oxidoreductase 2 (mqo2) CSB-YP839970SUL
CSB-EP839970SUL
CSB-BP839970SUL
CSB-MP839970SUL
CSB-EP839970SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartate 1-decarboxylase (panD) CSB-YP839971SUL
CSB-EP839971SUL
CSB-BP839971SUL
CSB-MP839971SUL
CSB-EP839971SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative surface protein MW2416 (MW2416), partial CSB-YP839972SUL
CSB-EP839972SUL
CSB-BP839972SUL
CSB-MP839972SUL
CSB-EP839972SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable nitrate transporter narT (narT), partial CSB-YP839973SUL
CSB-EP839973SUL
CSB-BP839973SUL
CSB-MP839973SUL
CSB-EP839973SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized HTH-type transcriptional regulator MW2284 (MW2284) CSB-YP839974SUL
CSB-EP839974SUL
CSB-BP839974SUL
CSB-MP839974SUL
CSB-EP839974SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Formimidoylglutamase (hutG) CSB-YP839975SUL
CSB-EP839975SUL
CSB-BP839975SUL
CSB-MP839975SUL
CSB-EP839975SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease accessory protein UreF (ureF) CSB-YP839976SUL
CSB-EP839976SUL
CSB-BP839976SUL
CSB-MP839976SUL
CSB-EP839976SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdopterin molybdenumtransferase (moeA) CSB-YP839977SUL
CSB-EP839977SUL
CSB-BP839977SUL
CSB-MP839977SUL
CSB-EP839977SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP839978SUL
CSB-EP839978SUL
CSB-BP839978SUL
CSB-MP839978SUL
CSB-EP839978SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable transglycosylase sceD (sceD) CSB-YP839979SUL
CSB-EP839979SUL
CSB-BP839979SUL
CSB-MP839979SUL
CSB-EP839979SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine-protein kinase rsbW (rsbW) CSB-YP839980SUL
CSB-EP839980SUL
CSB-BP839980SUL
CSB-MP839980SUL
CSB-EP839980SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized leukocidin-like protein 2 (MW1942) CSB-YP839981SUL
CSB-EP839981SUL
CSB-BP839981SUL
CSB-MP839981SUL
CSB-EP839981SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized RNA methyltransferase MW1838 (MW1838) CSB-YP839982SUL
CSB-EP839982SUL
CSB-BP839982SUL
CSB-MP839982SUL
CSB-EP839982SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Signal transduction protein TRAP (traP) CSB-YP839983SUL
CSB-EP839983SUL
CSB-BP839983SUL
CSB-MP839983SUL
CSB-EP839983SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein CrcB homolog 1 (crcB1), partial CSB-YP839984SUL1
CSB-EP839984SUL1
CSB-BP839984SUL1
CSB-MP839984SUL1
CSB-EP839984SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0354 protein MW1686 (MW1686) CSB-YP839985SUL
CSB-EP839985SUL
CSB-BP839985SUL
CSB-MP839985SUL
CSB-EP839985SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable tRNA sulfurtransferase (thiI) CSB-YP839986SUL
CSB-EP839986SUL
CSB-BP839986SUL
CSB-MP839986SUL
CSB-EP839986SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP839987SUL
CSB-EP839987SUL
CSB-BP839987SUL
CSB-MP839987SUL
CSB-EP839987SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP839988SUL
CSB-EP839988SUL
CSB-BP839988SUL
CSB-MP839988SUL
CSB-EP839988SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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