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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthomonas campestris pv. campestris 30S ribosomal protein S18 (rpsR) CSB-YP840090XAY
CSB-EP840090XAY
CSB-BP840090XAY
CSB-MP840090XAY
CSB-EP840090XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP840091XAY
CSB-EP840091XAY
CSB-BP840091XAY
CSB-MP840091XAY
CSB-EP840091XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Protein SlyX homolog (slyX) CSB-YP840092XAY
CSB-EP840092XAY
CSB-BP840092XAY
CSB-MP840092XAY
CSB-EP840092XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP840093XAY
CSB-EP840093XAY
CSB-BP840093XAY
CSB-MP840093XAY
CSB-EP840093XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Lipid-A-disaccharide synthase (lpxB) CSB-YP840094XAY
CSB-EP840094XAY
CSB-BP840094XAY
CSB-MP840094XAY
CSB-EP840094XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L19 (rplS) CSB-YP840095XAY
CSB-EP840095XAY
CSB-BP840095XAY
CSB-MP840095XAY
CSB-EP840095XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris UvrABC system protein A (uvrA), partial CSB-YP840096XAY
CSB-EP840096XAY
CSB-BP840096XAY
CSB-MP840096XAY
CSB-EP840096XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L32 (rpmF) CSB-YP840097XAY
CSB-EP840097XAY
CSB-BP840097XAY
CSB-MP840097XAY
CSB-EP840097XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP840098XAY
CSB-EP840098XAY
CSB-BP840098XAY
CSB-MP840098XAY
CSB-EP840098XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L15 (rplO) CSB-YP840099XAY
CSB-EP840099XAY
CSB-BP840099XAY
CSB-MP840099XAY
CSB-EP840099XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L2 (rplB) CSB-YP840100XAY
CSB-EP840100XAY
CSB-BP840100XAY
CSB-MP840100XAY
CSB-EP840100XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Elongation factor G (fusA), partial CSB-YP840101XAY
CSB-EP840101XAY
CSB-BP840101XAY
CSB-MP840101XAY
CSB-EP840101XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribose-phosphate pyrophosphokinase (prs) CSB-YP840102XAY
CSB-EP840102XAY
CSB-BP840102XAY
CSB-MP840102XAY
CSB-EP840102XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Glutamine--tRNA ligase (glnS), partial CSB-YP840103XAY
CSB-EP840103XAY
CSB-BP840103XAY
CSB-MP840103XAY
CSB-EP840103XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP840104XAY1
CSB-EP840104XAY1
CSB-BP840104XAY1
CSB-MP840104XAY1
CSB-EP840104XAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Protein translocase subunit SecA (secA), partial CSB-YP840105XAY
CSB-EP840105XAY
CSB-BP840105XAY
CSB-MP840105XAY
CSB-EP840105XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP840106XAY
CSB-EP840106XAY
CSB-BP840106XAY
CSB-MP840106XAY
CSB-EP840106XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP840107XAY
CSB-EP840107XAY
CSB-BP840107XAY
CSB-MP840107XAY
CSB-EP840107XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris ATP synthase subunit b (atpF), partial CSB-YP840108XAY
CSB-EP840108XAY
CSB-BP840108XAY
CSB-MP840108XAY
CSB-EP840108XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP840109XAY
CSB-EP840109XAY
CSB-BP840109XAY
CSB-MP840109XAY
CSB-EP840109XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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