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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Threonine--tRNA ligase (thrS), partial CSB-YP844166SUL
CSB-EP844166SUL
CSB-BP844166SUL
CSB-MP844166SUL
CSB-EP844166SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0271 protein MW1555 (MW1555) CSB-YP844167SUL
CSB-EP844167SUL
CSB-BP844167SUL
CSB-MP844167SUL
CSB-EP844167SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable rRNA maturation factor (MW1522) CSB-YP844168SUL
CSB-EP844168SUL
CSB-BP844168SUL
CSB-MP844168SUL
CSB-EP844168SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP844169SUL
CSB-EP844169SUL
CSB-BP844169SUL
CSB-MP844169SUL
CSB-EP844169SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chorismate synthase (aroC) CSB-YP844170SUL
CSB-EP844170SUL
CSB-BP844170SUL
CSB-MP844170SUL
CSB-EP844170SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus L-threonine dehydratase catabolic TdcB (tdcB) CSB-YP844171SUL
CSB-EP844171SUL
CSB-BP844171SUL
CSB-MP844171SUL
CSB-EP844171SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP844172SUL
CSB-EP844172SUL
CSB-BP844172SUL
CSB-MP844172SUL
CSB-EP844172SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative oligopeptide transport ATP-binding protein oppF2 (oppF2) CSB-YP844173SUL
CSB-EP844173SUL
CSB-BP844173SUL
CSB-MP844173SUL
CSB-EP844173SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Prephenate dehydrogenase (tyrA) CSB-YP844174SUL
CSB-EP844174SUL
CSB-BP844174SUL
CSB-MP844174SUL
CSB-EP844174SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA mismatch repair protein mutL (mutL), partial CSB-YP844175SUL
CSB-EP844175SUL
CSB-BP844175SUL
CSB-MP844175SUL
CSB-EP844175SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative zinc metalloprotease MW1145 (MW1145), partial CSB-YP844176SUL1
CSB-EP844176SUL1
CSB-BP844176SUL1
CSB-MP844176SUL1
CSB-EP844176SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Succinyl-CoA ligase [ADP-forming] subunit alpha (sucD) CSB-YP844177SUL
CSB-EP844177SUL
CSB-BP844177SUL
CSB-MP844177SUL
CSB-EP844177SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Orotate phosphoribosyltransferase (pyrE) CSB-YP844178SUL
CSB-EP844178SUL
CSB-BP844178SUL
CSB-MP844178SUL
CSB-EP844178SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0747 protein MW1060 (MW1060), partial CSB-YP844179SUL
CSB-EP844179SUL
CSB-BP844179SUL
CSB-MP844179SUL
CSB-EP844179SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP844180SUL
CSB-EP844180SUL
CSB-BP844180SUL
CSB-MP844180SUL
CSB-EP844180SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide deformylase (def) CSB-YP844181SUL
CSB-EP844181SUL
CSB-BP844181SUL
CSB-MP844181SUL
CSB-EP844181SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N5-carboxyaminoimidazole ribonucleotide synthase (purK) CSB-YP844182SUL
CSB-EP844182SUL
CSB-BP844182SUL
CSB-MP844182SUL
CSB-EP844182SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease htrA-like (MW0903), partial CSB-YP844183SUL1
CSB-EP844183SUL1
CSB-BP844183SUL1
CSB-MP844183SUL1
CSB-EP844183SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP844184SUL
CSB-EP844184SUL
CSB-BP844184SUL
CSB-MP844184SUL
CSB-EP844184SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP844185SUL
CSB-EP844185SUL
CSB-BP844185SUL
CSB-MP844185SUL
CSB-EP844185SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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