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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Corynebacterium glutamicum Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP847787DWY
CSB-EP847787DWY
CSB-BP847787DWY
CSB-MP847787DWY
CSB-EP847787DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP847788DWY
CSB-EP847788DWY
CSB-BP847788DWY
CSB-MP847788DWY
CSB-EP847788DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (glgE), partial CSB-YP847789DWY
CSB-EP847789DWY
CSB-BP847789DWY
CSB-MP847789DWY
CSB-EP847789DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP847790DWY
CSB-EP847790DWY
CSB-BP847790DWY
CSB-MP847790DWY
CSB-EP847790DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Fumarate hydratase class II (fumC) CSB-YP847791DWY
CSB-EP847791DWY
CSB-BP847791DWY
CSB-MP847791DWY
CSB-EP847791DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Bifunctional protein GlmU (glmU) CSB-YP847792DWY
CSB-EP847792DWY
CSB-BP847792DWY
CSB-MP847792DWY
CSB-EP847792DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Putative regulator of ribonuclease activity (Cgl0925, cg1055) CSB-YP847793DWY
CSB-EP847793DWY
CSB-BP847793DWY
CSB-MP847793DWY
CSB-EP847793DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L28 (rpmB) CSB-YP847794DWY
CSB-EP847794DWY
CSB-BP847794DWY
CSB-MP847794DWY
CSB-EP847794DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP847795DWY
CSB-EP847795DWY
CSB-BP847795DWY
CSB-MP847795DWY
CSB-EP847795DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Adenosylhomocysteinase (ahcY) CSB-YP847796DWY
CSB-EP847796DWY
CSB-BP847796DWY
CSB-MP847796DWY
CSB-EP847796DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 2-methylcitrate synthase 2 (prpC2) CSB-YP847797DWY
CSB-EP847797DWY
CSB-BP847797DWY
CSB-MP847797DWY
CSB-EP847797DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Bifunctional NAD (P)H-hydrate repair enzyme Nnr (nnr), partial CSB-YP847798DWY
CSB-EP847798DWY
CSB-BP847798DWY
CSB-MP847798DWY
CSB-EP847798DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum tRNA pseudouridine synthase A (truA) CSB-YP847799DWY
CSB-EP847799DWY
CSB-BP847799DWY
CSB-MP847799DWY
CSB-EP847799DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 30S ribosomal protein S13 (rpsM) CSB-YP847800DWY
CSB-EP847800DWY
CSB-BP847800DWY
CSB-MP847800DWY
CSB-EP847800DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L5 (rplE) CSB-YP847801DWY
CSB-EP847801DWY
CSB-BP847801DWY
CSB-MP847801DWY
CSB-EP847801DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L23 (rplW) CSB-YP847802DWY
CSB-EP847802DWY
CSB-BP847802DWY
CSB-MP847802DWY
CSB-EP847802DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L7/L12 (rplL) CSB-YP847803DWY
CSB-EP847803DWY
CSB-BP847803DWY
CSB-MP847803DWY
CSB-EP847803DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Porphobilinogen deaminase (hemC) CSB-YP847804DWY
CSB-EP847804DWY
CSB-BP847804DWY
CSB-MP847804DWY
CSB-EP847804DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Inositol 2-dehydrogenase (iolG) CSB-YP847805DWY
CSB-EP847805DWY
CSB-BP847805DWY
CSB-MP847805DWY
CSB-EP847805DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0233 membrane protein Cgl0040/cg0055 (Cgl0040, cg0055), partial CSB-YP847806DWY1
CSB-EP847806DWY1
CSB-BP847806DWY1
CSB-MP847806DWY1
CSB-EP847806DWY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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